Abstract：Objective To establish a method for the determination of glutamine in Glutamine Capsules by ultra-high performance liquid chromatography tandem mass spectrometry.Methods An ultra-high performance liquid chromatography tandem mass spectrometer was used.The column was Atiantis T3(1100 mm×2 mm,3 μm),and the column temperature was 38℃.The mobile phase was 0.1% formic acid solution∶acetonitrile,and flow rate was 0.30 mL/min.The content of glutamine in Glutamine Capsules was determined by multi reaction monitoring mode.Results The linear relationship of glutamine was good in the range,linear regression equation Y=4×10-6X+0.1336,RSD was 2.2%～4.8%,and the detection limit of glutamine was 9.8 ng/mL.Conclusion The method is simple,specific,accurate and sensitive,and can be used for the determination of glutamine in medicine and biological samples.
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