抗癌烷化剂三溴基丙酮酸对秀丽隐杆线虫衰老指标及寿命的影响

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摘要目的利用16SrRNA测序技术，探讨早期经验性应用抗生素不同疗程对早产儿日龄28 d肠道微生物的影响。方法选取2017年12月～2018年5月在广东省妇幼保健院新生儿科住院治疗的27例早产儿作为研究对象，其中使用青霉素+三代头孢菌素≤3d为短疗程组（G1组，9例），使用青霉素+三代头孢菌素≥7 d为长疗程组（G2组，18例）。收集28 d日龄的粪便标本，采用16Sr RNA测序技术分析菌群组成的变化。结果菌群的结构组成中，主要的菌门包括放线菌门、拟杆菌门、厚壁菌门、变形菌门，其中厚壁菌门含量最为丰富。G1组中的拟杆菌门（0.03%）和变形菌门（34.11%）与G2组（0.04%、14.23%）比较，差异无统计学意义（P＞0.05）。G1组和G2组的alpha多样性比较，差异无统计学意义（P＞0.05）。G1组中的β-变形菌纲（LDA=6.43，P=0.017）、丛毛单菌科（LDA=6.24，P=0.017）、皮杆菌科（LDA=6.60，P=0.016）、黄体杆菌属（LDA=6.06，P=0.026）和韦荣球菌属（LDA=7.51，P=0.049）高于G2组，差异有统计学意义（P＜0.05）。结论早期经验性、长期应用广谱抗生素可能会导致早产儿肠道正常菌群的定植和达优势化时间延迟。

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	王俊平
	王艳丽
	钟隽镌
	颜慧恒
	张永
	陈运彬

关键词 ：抗生素, 肠道菌群, 早产, 16S rRNA测序

Abstract：Objective To explore the influence of early empirical application of different treatment courses of antibiotic on the gutmicrobiota of28-day-old preterm infants through 16S rRNA sequencing technology.Methods From December 2017 to May 2018,a total of 27 cases of hospitalized preterm infants in GuangdongWomen and Children Hospitalwere enrolled,among them,penicillin+the third generation cephalosporin≤3dayswere selected as the short course group(the G1group,9 cases),penicillin+the third generation cephalosporin≥7 dayswere selected as the long course group(the G2group,18 cases).The fecal samplesatpostnatal28 dayswere collected,and 16S rRNA sequencing technology was used to analyze the changes ofmicrobial community diversity.Results In the structural composition of the flora,the main phylum included Actinobacteria,Bacteroidetes,Firmicutes,and Proteobacteria,among which pachytene was the most abundant,the thick-walled mycoplasma was the most abundant.There were no significant differences in the Bacteroidetes and Proteobacteria between the G1group(0.03%,34.11%)and the G2group(0.04%,14.23%)(P＞0.05).There was no statistical difference in the alpha diversity between the G1group and the G2group (P＞0.05).Betaproteobacteria(LDA=6.43,P=0.017),Comomamonadaceae(LDA=6.24,P=0.017),Dermabacteraceae(LDA=6.60,P=0.016),Luteibacter(LDA=6.06,P=0.026)and Veillonella (LDA=7.51,P=0.049)in the G1group were higher than those in the G2group,there were statistical differences (P＜0.05).Conclusion Early empirical and long-term application of broad-spectrum antibiotic may lead to the colonization of normal intestinal flora and the delay of the advantage of time in preterm infants.

Key words： Preterm infant Gutmicrobiota Antibiotic 16S rRNA sequencing

基金资助:广东省科技计划项目（2017ZC0324）；广东省广州市科技计划项目（201804010363）；广东省广州市科技计划项目（201804010370）

引用本文:

王俊平; 王艳丽; 钟隽镌; 颜慧恒; 张永; 陈运彬. 抗癌烷化剂三溴基丙酮酸对秀丽隐杆线虫衰老指标及寿命的影响[J]. 中国当代医药, 2020, 27(17): 8-11.
WANG Jun-ping; WANG Yan-li; ZHONG Jun-juan; YAN Hui-heng; ZHANG Yong; CHEN Yun-bin. Influence of early use of different courses of antibiotic on the gutm icrobiota of 28-day-old preterm infants. 中国当代医药, 2020, 27(17): 8-11.

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