The effect of Pirfenidone combined with Nintedanib treating pulmonary fibrosis in mice
WANG Xue1 LI Qiao-ling2 GAO Yun-yun2 DENG Wen2▲
1. Clinical Medical College, Taishan Medical University, Shandong Province, Tai′an 271000, China；
2. College of Basic Medical Sciences, Taishan Medical University, Shandong Province, Tai′an 271000, China
Abstract：Objective To explore the effect and method of Pirfenidone combined with Nintedanib treating pulmonary fibrosis in mice. Methods All of 90 male Kunming mice aged 6-8 weeks were selected, After 1 week of adaptive feeding, and among them, 10 mice were randomly selected as control group, and the other 80 mice were treated with Bleomycin to establish pulmonary fibrosis model. The pulmonary function of the mice was measured by Buxco animal pulmonary function analysis system. The Penh value increased to more than 1.5, and the model was established successfully. 40 model mice were selected randomly from successful mice modeling and randomly divided into control group (0.1 ml normal saline, 3 times a day), Pirfenidone group (400 mg/kg, 3 times a day), Nintedanib group (150 mg/kg,2 times a day), Pirfenidone (400 mg/kg, 3 times a day) combined with Nintedanib group (150 mg/kg, 2 times a day).The mice were gavaged for 3 weeks. Penh value was measured using the above-mentioned method. Then the mice were sacrificed after anaesthetized. The alveolar lavage fluid was collected and centrifuged, then the cells were obtained. After Swiss-Giemsa staining, the white cells in alveolar lavage fluid of mice were counted under inverted microscope.Results A total of 80 mice were modeled and 50 mice (62.5%) were successfully modeled. After treatment, the Penh value in Pirfenidone group combined with Nintedanib group were lower than those in Pirfenidone group (P＜0.05),Nintedanib group (P＜0.05) and control group (P＜0.01). The leukocyte of Pirfenidone combined with Nintedanib group was fewer than that of Pirfenidone group (P＜0.05), Nintedanib group (P＜0.05) and control group (P＜0.01). Conclusion The therapeutic effect of Pirfenidone combined with Nintedanib is the best, which lays a foundation for the clinical use of this method in the treatment of pulmonary fibrosis.
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