杜仲、山楂和陈皮混合汤剂对营养型肥胖大鼠减肥降脂的作用

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摘要目的探究陈皮、杜仲以及山楂3种中草药混合汤剂对高脂高糖诱导的肥胖大鼠的减肥降脂作用。方法选取高脂饲料饲养的50只Wistar雄性大鼠作为研究对象，制备肥胖大鼠模型。将造模后的大鼠随机分为模型组、阳性对照组、低剂量组、中剂量组、高剂量组，每组各10只。另外选取给予基础饲料饲养的10只大鼠作为对照组。低剂量组、中剂量组、高剂量组每天分别灌胃1 ml/100 g、2 ml/100 g、4 ml/100 g的中草药混合汤剂，阳性对照组大鼠灌胃奥利司他悬浮液60 mg/（kg·d）。均连续灌胃8周。模型组和对照组以相同方法灌胃生理盐水。用酶联免疫法检测各组血清中三酰甘油（TG）、总胆固醇（TC）、高密度脂蛋白胆固（HDL-C）、低密度脂蛋白胆固（LDL-C）的含量及肝脏中脂肪酸合成酶（FAS）、提高酰基辅酶A氧化酶（ACO）的含量，比较各组大鼠的各类肠道菌群数量。结果模型组大鼠的体重、Lee′s指数、TG、TC、LDL-C、FAS含量均显著高于对照组，HDL-C、ACO低于对照组，差异均有统计学意义（P<0.05）。高剂量组大鼠的体重和Lee′s指数、TG、TC、LDL-C、FAS含量显著低于模型组，HDL-C、ACO水平显著高于模型组，差异均有统计学意义（P<0.05）。高剂量组大鼠肠道内的双歧杆菌、乳酸菌、肠球菌数量多于模型组，大肠埃希菌数量少于模型组，差异均有统计学意义（P<0.05）。高剂量组大鼠的Lee′s指数、TG、TC、LDL-C、FAS、ACO含量与阳性对照组比较，差异均无统计学意义（P>0.05）。结论陈皮、杜仲和山楂3种草药混合汤剂可以抑制FAS活性、促进ACO活性、提高益生菌数量，对高脂饮食诱导的肥胖大鼠具有显著的减肥降脂作用。

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	林园园
	熊义涛

关键词 ：肥胖, 杜仲, 山楂, 三酰甘油, 酰基辅酶A氧化酶

Abstract：ObjectiveTo investigate the effect of Tangerine Peel,Eucommia and Hawthorn mixture decoction on reducing fat in obese rats induced by high fat diet.MethodsA total of 50 Wistar male rats who fed with high fat diet to establish the rat model of obesity were selected as subjects,which were divided into model group,positive group,low-dose group,middle-dose group and high-dose group randomly,10 rats in each groups.In addition,10 normal feeding rats were selected as the control group.The low-dose group,the middle-dose group and the high-dose group were treated with 1 ml/100 g,2 ml/100 g,4 ml/100 g herbal mixture decoction respectively each day.The rats in the positive group were intragastric Orlistat Suspension 60 mg/kg by gavage.All were treated continuously for 8 weeks.The model group and the control group were given normal saline by gavage with the same method.The content of serum triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C)in serum and the contend of fatty acid synthase(FAS),acyl coenzyme A oxidase(ACO)in liver were detected by enzyme-linked immunosorbent assay.The number of intestinal flora of rats in each group were compared.ResultsThe weight,Lee′s index,TG,TC,LDL-C and FAS contents of the rats in the model group were significantly higher than those in the control group,and HDL-C and ACO of the rats in the model group were lower than those in the control group,the differences were statistically significant(P<0.05).The weight and Lee′s index,TG,TC,LDL-C and FAS contents of the rats in the high-dose group were significantly lower than those in the model group,and the levels of HDL-C and ACO of the rats in the high-dose group were significantly higher than those in the model group,the differences were statistical-ly significant(P<0.05).The number of bifidobacterium,lactobacillus and enterococcus in the intestinal tract of rats in high-dose group were higher than those in the model group,and the number of escherichia coli was less than that of the model group,the differences were statistically significant(P<0.05).There were no statistically significant differences in Lee′s index,TG,TC,LDL-C,FAS and ACO of rats in high-dose group compared with the positive group　(P>0.05).ConclusionThe three herbs mixed decoction of Tangerine Peel,Eucommia and Hawthorn can inhibit the activity of FAS,promote the activity of ACO and increase the number of probiotics.It had a significant effect on reducing weight and blood lipids in obese rats induced by high fat diet.

Key words： Obesity Eucommia Hawthorn Triglyceride Acyl coenzyme A oxidase

作者简介: 林园园（1976-），女，主管中药师，研究方向：中药

引用本文:

林园园　；熊义涛. 杜仲、山楂和陈皮混合汤剂对营养型肥胖大鼠减肥降脂的作用[J]. 中国当代医药, 2018, 25(17): 20-23转28.
LIN Yuan-yuan；　XIONG Yi-tao. Effects of Haw thorn,Eucomm ia and Tangerine Peel m ixture decoction on reducing fat in obese rats induced by high fat diet. 中国当代医药, 2018, 25(17): 20-23转28.

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http://dangdaiyiyao.com/CN/ 或 http://dangdaiyiyao.com/CN/Y2018/V25/I17/20

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