Detection and genetic therapy significance of PTEN,p14ARFand Survivin in lung carcinoma
REN Li-juan1 LIU Ya-li2 HOU Mei-feng1 DING Yan-long1
1.Department of ICU，Inner Mongolia Autonomy No 4 Hospital，Huhhot 010080，China；
2.No 1 Department of Medicine，General Hospital of the Armed Police Force in Inner Mongolia，Huhhot 010041，China
Abstract：Objective To study the expression and the relationship of PTEN，p14ARFand Survivin in lung carcinoma and offer more scientific theoretic basis for treatment and diagnosis of lung carcinoma.Methods 87 cases of primary bronchogenic carcinoma tissues and 13 cases normal lung tissues in our hospital from January 2009 to December 2015 were detected by SP immunohistochemical method，and their expression and correlation were studied.Results The positive expression of PTEN，p14ARFwere higher in normal lung tissue than primary bronchogenic carcinoma，the expression of Survivin was in contrast.The expression of PTEN，p14ARFwere reduced with poorly，moderately and welldifferentiated group，by contrast，the expression of Survivin was increasing（P＜0.01）.The expression of PTEN，p14ARFwere reduced，while the expression of Survivin was increasing with clinical stages enlarging in clinical stages group，PTEN，p14ARFand Survivin among stageⅠ，Ⅱ，Ⅲ，Ⅳhad statistical difference（P＜0.01）.Among PTEN，p14ARFand Survivin were correlatively analyzed in lung carcinoma.There is a positive correlation between PTEN and p14ARF（r=0.6489，P＜0.01），with negative correlation between Survivin and PTEN（r=-0.5743，P＜0.01）or Survivin and p14ARF（r=-0.6860，P＜0.01）.The three gene are associated with the primary bronchogenic carcinoma.Conclusion PTEN，p14ARF，Survivin play a combined role in the apoptosis and proliferation of primary bronchogenic carcinoma.It is important to improve our treatment and diagnosis of primary bronchogenic carcinoma.
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