糖原合成酶激酶-3与线粒体分裂蛋白在减轻大鼠心肌缺血再灌注损伤作用中的关系

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摘要目的探讨糖原合成酶激酶-3（GSK-3β）与线粒体分裂蛋白（Drp-1）在大鼠心肌保护中的关系。方法选取清洁健康雄性SD大鼠60只，体重250～300 g，随机分为4组（n=15）：缺血再灌注组（IR组）、缺血再灌注+Mivi-1处理组（M组）、缺血再灌注+SB216763处理组（B组）、缺血再灌注+Mivi-1处理+SB216763处理组（D组）。采用结扎大鼠左冠状动脉前降支30 min，再灌注120 min的方法制备大鼠心肌缺血再灌注（MRIR）模型。M组再灌注前1 min静注 Mivi-1（1.2 mg/kg）；B 组再灌注前 1 min静注 SB216763（0.2 mg/kg）；D 组再灌注前静注 Mivi-1及 SB216763。腹主动脉取血检测血清肌钙蛋白（cTnI）浓度；取心肌组织，TUNEL法检测细胞凋亡情况，计算凋亡指数（AI）；观察心肌病理学结果；Western blot法检测caspase-3表达水平。结果与IR组比较，M、B、D组上述指标下调（P<0.05）；与D组比较，M、B组caspase-3浓度、AI、血清cTnI浓度上调，差异均有统计学意义（P<0.05）。结论GSK-3β与Drp-1在大鼠心肌保护作用中并非单一依赖关系。

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	罗维昊
	贺建东
	韩冲芳

关键词 ：心肌, 心肌再灌注损伤, 糖原合成酶激酶-3

Abstract：ObjectiveTo investigate the relationship between GSK-3β and Drp-1 in the myocardium protection in rats.Methods60 healthy male SD rats,weighing 250-300 g,randomly divided into 4 groups　(n=15)：Ischemia reperfusion group(group IR),Ischemia reperfusion+Mivi-1 treatment group(group M),Ischemia reperfusion+SB216763 treatment group(group B),and Ischemia reperfusion+Mivi-1 treatment group+SB216763 treatment group(group D).The myocardial ischemia-reperfusion injury model was established by ligating the LAD for 30 min and reperfusion for 120 min.Mdivi-1(1.2 mg/kg)was given at 1 min before reperfusion in group M by intravenous injection.SB216763(0.2 mg/kg)was given at 1 min before reperfusion in group B by intravenous injection.Both of them were given at 1 min before reperfusion in group D by intravenous injection.cTnI concentration was detected from abdominal aorta blood.Myocardial specimens were obtained from the apex for determination of the AI by TUNEL.Myocardial pathology were observed and the expression of caspase-3 was detected by Western blot.ResultsCompared with IR group,the levels of caspase-3,AI and myocardial injury in M,B and D groups were down-regulated(P<0.05).Compared with D group,the levels of caspase-3,AI and myocardial injury were increased in M and B group the differences were statistical significant(P<0.05).Conclusion The relationship between GSK-3β and Drp-1 of the myocardium protection in rats is not a single dependency.

Key words： Myocardium Myocardial ischemia-reperfusion GSK-3&beta

基金资助:山西省卫生和计划生育委员会科研课题（2015002）

引用本文:

罗维昊；　贺建东；　韩冲芳. 糖原合成酶激酶-3与线粒体分裂蛋白在减轻大鼠心肌缺血再灌注损伤作用中的关系[J]. 中国当代医药, 2018, 25(17): 12-15.
LUO Wei-hao；HE Jian-dong；HAN Chong-fang2▲. The relationship between GSK-3 beta and Drp-1 in alleviating myocardial ischem ia reperfusion injury in rats. 中国当代医药, 2018, 25(17): 12-15.

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http://dangdaiyiyao.com/CN/ 或 http://dangdaiyiyao.com/CN/Y2018/V25/I17/12

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