Protection mechanism of intermittent hypoxia on heart ischemia/reperfusion injury in rats
WANG Yuan-yuan1 CAO Jian2▲ WAN Jian-hua1
1.Department of Cardiology,the Third Hospital of Nanchang City,Jiangxi Province,Nanchang 330009,China;
2.Department of Anesthesiology,the Second Affiliated Hospital of Nanchang University,Jiangxi Province,Nanchang 330006,China;
3.Department of Anesthesiology,Hospital of Traditional Chinese Medicine of Jingdezhen City,Jiangxi Province,Jingdezheng 333000,China
Abstract：Objective To study the expression of phosphatidylinositide 3-kinase signaling pathway(PI3K),hypoxia-inducible factor 1α (HIF-1α),vascular endothelial growth Factor-2(VEGF-2)and to studied the correlation protection mechanism of intermittent hypoxia on heart ischemia/reperfusion(I/R)injury in rats.Methods 72 rats were randomly divided into the sham-operated group(n=18),the myocardial I/R group(n=18),the myocardial I/R+intermittent hypoxia(IH)group(I/R+IH)(n=18)and the myocardial I/R+IH+phosphatidylinositide 3-kinase(PI3K)inhibitor(wortmannin)group(IH+I/R+PI3K inhibitor group)(n=18).Mice were exposed to cycles to establish the IH model.I/R model was established by ligating the left anterior descending coronary artery.The MDA and GSH of cardic muscle tissues,and the serum biomarkers CK-MB in the four groups were detected.The LV end-diastolic volume(LVEDV),LV end-systolic(LVESV),LV ejection fraction(LVEF)of the rats by echocardiography after 8 weeks were assessed.The expression of phosphorylation of protein kinase B (Akt),HIF-1α,VEGF-2 was analyzed by Westen-blot.Results The level of MDA,CK-MB,LVEDV,LVESV of the I/R group was higher than that of the sham-operated group,the value of GSH,LVEF and the expression of AKt,HIF-1α,VEGF-2 of the I/R group was lower than that of the sham-operated group,with significant difference (P＜0.05).The level of MDA,CK-MB,LVEDV,LVESV of the I/R+IH group was lower than that of the I/R group,the value of GSH,LVEF and the expression of AKt,HIF-1α,VEGF-2 of the I/R+IH group was higher than that of the I/R group,with significant difference(P＜0.05).The level of MDA,CK-MB,LVEDV,LVESV of the I/R+IH+PI3K inhibitor group was higher than that of the sham-operated group,the value of GSH,LVEF and the expression of AKt,HIF-1α,VEGF-2 of the I/R+IH+PI3K inhibitor group was lower than that of the sham-operated group,with significant difference(P＜0.05).Conclusion The PI3K/Akt pathway and the expression of HIF-1α,VEGF-2 is important for IH-induced cardioprotection and may represent aviable target to reduce myocardial I/R injury.
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