Abstract：To establish thin layer and high performance liquid phase determination methods for Corydalis Rhizoma Chewable Tablets.Methods The silica gel G plate was prepared with 1% NaOH,and the Corydalis Rhizoma Chewable Tablets were identified by tetrahydropalmatine,with n-hexane-chloroform-methanol (7.5：4：1) as the developing agent.It was determined by reversed phase high performance liquid chromatography (RP-HPLC).The column was Hypersil BDS C18 (5 μm,4.6 mm×150 mm),the mobile phase was methanol-0.1% phosphoric acid (pH adjusted to 6.0 with triethylamine) (60：40),and the detection wavelength was 280 nm.Results The thin layer of Corydalis Rhizoma Chewable Tablets was identified and placed under ultraviolet light (365 nm).In the chromatogram of the test sample,the same color fluorescent spot was displayed at the same position as the reference substance,and the negative control did not have this spot.The content of tetrahydropalmatine in the Corydalis Rhizoma Chewable Tablets was determined by HPLC.The linear range of tetrahydropalmatine was 0.0832-0.4160 μg,r=0.9997,and the average recovery rate was 99.94% (RSD=0.52%).Conclusion The qualitative and quantitative quality control methods of the Corydalis Rhizoma Chewable Tablets are easy to operate with good reproducibility and high specificity,and can be used as quality control methods.
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