ObjectiveTo investigate the clinical effects of ultrasound-guided transretal prostatic biopsy(TPB)with 10-12 cores in diagnosis of prostate cancer.Methods One hundred and twenty cases of highly-suspected prostate cancer from October 2015 to October 2017 were selected,and they were divided into two groups by random number table,60 cases in each group.The control group was given ultrasound-guided TPB with 6-cores,while the experimental group was given ultrasound-guided TPB with 10-12 cores.The positive detection rate of prostate cancer by two detection methods were compared between the two groups.The postoperative complications of two groups were recorded.Results The success rate of biopsy in two groups were 100.00%,the positive detection rate of prostate cancer in experimental group was 45.00%(27/60),which was higher than 20.00%(12/60)of control group(P＜0.05).The incidence of complications after biopsy in experimental group and control group were 61.67%(37/60)and 60.00%(36/60),there was no statistical difference(P＞0.05).Conclusion Ultrasound-guided TPB with 10-12 cores in detection of prostate cancer can increase clinical diagnosis rate and not add the incidence of complications after biopsy,and it is safe and reliable,which can be applied in screening of prostate cancer.