Objective To explore the effect of thermal insulation nursing on surgical stress and anesthesia recovery with undergoing laparoscopic cholecystectomy (LC) under general anesthesia.Methods A total of 80 cases with cholecystolithiasis and/or cholecystitis undergoing LC admitted to our hospital from March 2018 to September 2019 were selected and divided into two groups according to random number table method, 40 cases in each group.The control group implemented routine nursing.The observation group was given the heat preservation nursing, then the body temperature measurement results during anesthesia recovery (when anesthesia stopped, when entering the recovery room and when entering the recovery room for 15 min) were compared.The changes of stress-related factors during anesthesia recovery were compared between the two groups.The related indexes of anesthesia awakening between the two groups were compared：the time of opening eyes, the time of assisted ventilation by anesthesia machine, time of tracheal catheter removal and the total time of stay in resuscitation room.The sedation score standard for anaesthetic awakening (Ramsay)change trend was compared between the two groups during anesthesia recovery period (when anesthesia stopped, when entering the recovery room and when entering the recovery room for 15 min).Results The body temperature in the observation group was higher than that in the control group (when anesthesia was stopped, when entering the resuscitation room and when entering the resuscitation room for 15 min), the difference was statistically significant (P<0.05).The stress-related factor malondialdehyde (MDA) during anesthesia and resuscitation was lower than that of the control group, the difference was statistically significant (P<0.05), the level of superoxide dismutase (SOD) was higher than that of the control group, the difference was statistically significant (P<0.05).In the related indexes of anesthesia recovery, the opening time of eyes, the assistant ventilation time of anesthesia machine, time of extubation and the total stay time in the resuscitation room in the observation group were shorter than those in the control group, the differences were statistically significant (P<0.05).The Ramsay score in the observation group was higher than that in the control group (when anesthesia was stopped, when anesthesia entered the resuscitation room and when anesthesia entered the resuscitation room for 15 min), the difference was statistically significant (P<0.05).Conclusion For patients undergoing LC under general anesthesia, real-time thermal insulation nursing can effectively maintain body temperature stability and reduce stress response and promote anesthesia recovery.