雌激素拮抗剂联合塞来昔布治疗家兔膝关节创伤性骨化性肌炎的实验研究

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摘要目的探讨雌激素拮抗剂他莫昔芬联合塞来昔布对家兔膝关节创伤性骨化性肌炎的预防作用。方法将30只家兔采用随机数字表法分为A、B、C三组，采用膝关节强力被动牵拉、暴力按摩后固定复制膝关节创伤性骨化性肌炎模型；A组采用生理盐水对照，B组采用塞来昔布治疗，C组采用他莫昔芬及塞来昔布治疗，连续给药9周。造模第1、5、9周后比较各组关节肿胀程度，第5、9周后比较各组骨化性肌炎程度。结果各组动物造模1周后关节周径均大于造模前（A组：t=7.14，P=0.000；B组：t=5.29，P=0.000；C组：t=4.86，P=0.001）；A组关节周径大于C组（t=3.96，P=0.002）、B组（t=3.57，P=0.003），C组关节周径小于B组（t=2.48，P=0.007）；造模5周后各组关节周径均小于造模1周后（A组：t=3.26，P=0.005；B组：t=2.94，P=0.004；C组：t=2.58，P=0.004），A组关节周径大于B组（t=5.19，P=0.000）、C组（t=6.10，P=0.000），C组关节周径小于B组（t=2.84，P=0.003）；造模9周后A组及B组周径均大于造模5周后（A组：t=1.96，P=0.010；B组：t=1.84，P=0.013），C组周径小于造模5后（t=2.29，P=0.008），A组关节周径大于C组（t=3.82，P=0.001）、B组（t=3.14，P=0.002），C组关节周径小于B组（t=4.29，P=0.000）。造模5、9周后各组间骨化性肌炎分级比较，差异均有统计学意义（5周后：H=7.29，P=0.001，9周后：H=17.16，P=0.000）；各时间点B组及C组骨化性肌炎分级低于A组（B组，5周后：U=4.26，P=0.004，9周后：U=8.46，P=0.000；C组，5周后：U=5.14，P=0.002，9周后：U=9.17，P=0.000），C组低于B组（5周后：U=4.26，P=0.004，9周后：U=4.89，P=0.003）。结论他莫昔芬联合塞来昔布能有效减缓创伤性骨化性肌炎的进展，改善预防效果。

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关键词 ：雌激素拮抗剂, 骨化性肌炎, 塞来昔布, 他莫昔芬

Abstract：Objective To investigate preventive effect of estrogen antagonist Tamoxifen combined with Celecoxib in the rabbit with knee joint traumatic myositis ossificans.Methods 30 rabbits were randomly divided into group A,B and C, traumatic myositis ossificans model of knee joint were make with knee joint fixed after strong passive stretch and violent massage,and rabits in group A were given the normal saline B were treated with Tamoxifen and Celecoxib and the group C were treated with Tamoxifen and Celecoxib,rabits in the group,all rabits were treated for 9 weeks.The swelling degree of knee joint were compared among three groups at 1,5,9 weeks after making model,degree of myositis ossificans were compared among three groups at 5,9 weeks after making model.Results Circumference of knee joint at 1 week after making model than was larger than before making model among three groups (group A:t=7.14,P=0.000;group B:t= 5.29,P=0.000;group C:t=4.86,P=0.001),and the group A was larger than that of the group C (t=3.96,P=0.002)and group B(t=3.57,P=0.003),and the group C was less than that of the group B(t=2.48,P=0.007);circumference of knee joint 5 week after making model was less than 1 week after making model among three groups(group A:t=3.26,P=0.005;group B:t= 2.94,P=0.004;group C:t=2.58,P=0.004),circumference of knee joint in group A was larger than that in the group B (t= 5.19,P=0.000)and group C(t=6.10,P=0.000),and the group B was larger than that in group C(t=2.84,P=0.003);9 week after making model,circumferences of knee joint of group A and group B were larger than those at 5 weeks after making model(group A:t=1.96,P=0.010;group B:t=1.84,P=0.013),and the group C was less than that at 5 weeks after making model(t=2.29,P=0.008),circumference of knee joint of the group A was larger than that in the group C(t=3.82,P=0.001) and group B(t=3.14,P=0.002),and group C was less than that in the group B(t=4.29,P=0.000).Degree of myositis ossificans at 5,and 9 week after making model was different among three groups(5 weeks:H=7.29,P=0.001,9 weeks:H=17.16, P=0.000),and degree of myositis ossificans in group B and group C was lower than that in the group A at different time point(group B,5 weeks:U=4.26,P=0.004,9 weeks:U=8.46,P=0.000;group C,5 weeks:U=5.14,P=0.002,9 weeks:U=9.17,P=0.000),and group C was lower than that in the group B(5 weeks:U=4.26,P=0.004,9 weeks:U=4.89,P=0.003).Conclusion Estrogen antagonist Amoxifen combined with Celecoxib can effectivly control the progress of myositis ossificans,and improve effect of prevention.

Key words：

Estrogen antagonists')" href="#">">Estrogen antagonists Myositis ossificans Celecoxib Tamoxifen

引用本文:

韩峰;卢成华;易力;姜虹. 雌激素拮抗剂联合塞来昔布治疗家兔膝关节创伤性骨化性肌炎的实验研究[J]. 中国当代医药, 2017, 24(9): 12-14.
HAN Feng;LU Cheng-hua;YI Li;JIANG Hong. Experimental study of estrogen antagonist combined with celecoxib in the treatment of rabbit with knee joint traumatic myositis ossificans. 中国当代医药, 2017, 24(9): 12-14.

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http://dangdaiyiyao.com/CN/ 或 http://dangdaiyiyao.com/CN/Y2017/V24/I9/12

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