Study of control method and standard for Strychnine in Shengnong Zhengtong Paste
GAN Shu-guang1 HU Ji-di2 CAO Yue2 XIAO Long-gao2 XU Shi-qiang2▲
1.Huangshi Yanwu Pharma.Co.,Ltd.in Hubei Province,Huangshi 435000,China;
2.Department of Pharmacy,Medical College of Wuhan University of Science and Technology in Hubei Province,Wuhan 430065,China
Abstract：ObjectiveTo establish a content determination method of Strychnine in Shengnong Zhengtong Paste,and to set its limited quantity standard.MethodsC18chromatographic column was used,and the mobile phase was phase A (acetonitrile)∶phase B (0.01 mol/L heptane sodium sulfonate and 0.02 mol/L KH2PO4of equal mixing solution,with 10% H3PO4to adjust pH value to 2.8)=21∶79.The flow rate was 1.0 ml/min,the column temperature was 30℃,and the detection wavelength was 260 nm.ResultsThe linear range was 54.048-2026.8 ng,r=0.9996,and the reproducibility and precision of this method was good (RSD＜2.0%).The limited quantity scheduled having maqianzi of 0.20-1.00 mg every 100 square centimeters calculating with Strychnine (C21H22N2O2).ConclusionThe method is simple,accurate and reproducible.It can be used for the determination of Strychnine in Shengnong Zhengtong Paste.Its limited quantity is objective and rational to ensure the safety of drug use.
甘曙光;胡吉迪;曹越;肖龙高;徐诗强. 神农镇痛膏中士的宁的控制方法及标准研究[J]. 中国当代医药, 2017, 24(15): 93-96.
GAN Shu-guang;HU Ji-di;CAO Yue;XIAO Long-gao;XU Shi-qiang. Study of control method and standard for Strychnine in Shengnong Zhengtong Paste. 中国当代医药, 2017, 24(15): 93-96.
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