Identification of Saposhnikovia divaricata and its adulterants based on DNA barcode(ITS2)
LIN Hao1 QIAN Jie-ying2 LI Si-lu3 OU Hui-hong1 XIA Hai-shan1，4 LIU Yong1，4▲
1.School of Pharmac，Guangdong Medical University，Dongguan 523808，China；
2.Department of Biochemistry and Molecular Biology，Guangxi Medical University，Nanning 530021，China；
3.School of Basic Medicine，Guangdong Medical University，Dongguan 523808，China；
广东省中医药局科研项目（20151264，20161139）；广东省教育厅青年创新人才项目（2014KQNCX100）；广东医科大学国家级大学生创新创业训练计划项目（201510571 020）；广东医科大学省级大学生创新创业训练计划项目（201510571046，201510571079）；广东医学院科研基金项目（M2015015）；广东医学院大学生创新创业训练计划项目（XJ105711528，XJ105711443，XJ105711449，XJ105711453）；广东医学院大学生创新实验项目（2015ZZDM004）4.Guangdong Key Laboratory for Research and Development of Natural Drugs，Zhanjiang 524023，China
Abstract：Objective To identify Saposhnikovia divaricata and its adulterants by using ITS2 sequence analysis and to secure medication safety.Methods ITS2 sequences of Saposhnikovia divaricata and its adulterants were downloaded from GenBank，MEGA 6.06 software was used for sequence analysis to summarize GC content，variation sites，estimate intra-and inter-specific genetic distances，and built NJ trees.Moreover，the secondary structure of ITS2 was predicted by using the ITS2 database websites.Results ITS2 sequence analysis and genetic distances calculation showed significant difference in the samples.Further more，NJ tree and secondary structure results could distinctly differentiate quality product and adulterants.Conclusion ITS2 can effectively identify Saposhnikovia divaricata and its adulterants，as a result，it can be used for rapid identification of Chinese medicinal materials.
林好;钱洁颖;李斯璐;欧辉鸿;夏海珊;刘镛. 基于DNA条形码（ITS2）的中药材防风与其混伪品的鉴定[J]. 中国当代医药, 2016, 23(15): 4-7.
LIN Hao;QIAN Jie-ying;LI Si-lu;OU Hui-hong;XIA Hai-shan;LIU Yong. Identification of Saposhnikovia divaricata and its adulterants based on DNA barcode(ITS2). 中国当代医药, 2016, 23(15): 4-7.
Schultz J，Muller T，Achtziger M，et al.The internal transcribed spacer 2 database—a web server for（not only）low level phylogenetic analyses［J］.Nucleic Acids Res，2006，34 （Web Server issue）：W704-W707.
［10］
Tamura K，Nei M，Kumar S.Prospects for inferring very large phylogenies by using the neighbor-joining method［J］. Proc Natl Acad Sci U S A，2004，101（30）：11030-11035.
［11］
Gao T，Yao H，Song J，et al.Identification of medicinal plants in the family Fabaceae using a potential DNA barcode ITS2［J］.J Ethnopharmacol，2010，130（1）：116-121.
［12］
Kekkonen M，Hebert PD.DNA barcode-based delineation of putativespecies：efficientstartfortaxonomicworkflows［J］. Mol Ecol Resour，2014，14（4）：706-715.
［13］
Xu C，Dong W，Shi S，et al.Accelerating plant DNA barcode reference library construction using herbarium specimens：improved experimental techniques［J］.Mol Ecol Resour，2015，15（6）：1366-1374.
［14］
Ankenbrand M，Keller A，Wolf M，et al.ITS2 databaseⅤ：Twice as much［J］.Mol Biol Evol，2015，32（11）：3030-3032.
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