|
|
|
| To explore the PCR-RFLP in testing the relationship between methylenetetrahydrofolate reductase gene polymorphism and the pathogenesis of pre eclampsia |
| JIANG Xin SHU Jun-jun SHU Ru-ming GONG Dan YAN San-hua LI Li |
| Department of Obstetrics and Gynecology,the Third Hospital affiliated to Nanchang University,Jinagxi Province,Nanchang 330008,China |
|
|
|
|
Abstract ObjectiveTo investigate the restriction fragment length polymorphism polymerase chain reaction(cleaved amplification polymorphism sequence-tagged sites,PCR-RFLP)in testing the relationship between methylenetetrahydrofolate reductase gene polymorphism and the pathogenesis of pre eclampsia,provide the basis for the prevention and treatment of preeclampsia.Methods100 preeclampsia (the case group)and 500 healthy pregnant women (the control group)from May 2016 to June 2017 treated in our hospital were selected as subjects to conduct polymorphism analysis of methylenetetrahydrofolate reductase gene and detect of gene polymorphism.Blood biochemical indexes were analyzed,basic data was investigated and factors were analyzed.ResultsAll cases were successfully sequenced in the case group,and 498 cases were successfully sequenced in the control group.The genotype frequency and allele frequency of the case group were compared,and the differences were statistically significant(P＜0.05).The the number of junior high school degree and below level,planned pregnancy,planned to control diet and nutritional supplements,folic acid,vitamin D supplements,the proportion of malnutrition were compared,and the differences were statistically significant(P＜0.05).APPT in the case group was lower than that in control group,and Hcy was higher than that in control group,the differences were statistically significant(P＜0.05).Logistic regression analysis was used for multivariate analysis.The results showed that allele TT,folic acid supplementation and planned exercise during pregnancy were independent risk factors(P＜0.05).There was significant correlation between TT type and Hcy and APPT(P＜0.05).ConclusionMethylenetetrahydrofolate reductase gene polymorphisms are associated with preeclampsia and have independent effects,which may be related to the elevation of Hcy.
|
|
|
|
|
|
| [1] |
谢幸，苟文丽.妇产科学（第八版）[M].北京：人民卫生出版社，2013，64-71.
|
| [2] |
黄萍，张林艳，姜文静.早发型重度子痫前期的诊治进展[J].中国妇产科临床杂志，2014，15（1）：92-95.
|
| [3] |
邓文秋.正常妊娠与重度子痫前期孕妇血常规参数的孕期变化对比[J].中国妇幼保健，2013，28（6）：922-923.
|
| [4] |
黄丽媛，芦文丽，孙忠.我国妊娠高血压综合征相关危险因素 Meta 分析[J].中国预防医学杂志，2012，13（3）：225-227.
|
| [5] |
安妮，钱金栿，张阳，等.MTHFR基因C677T多态性与妊娠期高血压相关性的Meta分析[J].中国全科医学，2016，19（S1）：362-367.
|
| [6] |
孙玲玲，方芳，荆洪英，等.MTHFRC677T基因多态性与复发性自然流产相关性的Meta分析[J].黑龙江医药科学，2015，38（6）：52-55.
|
| [7] |
Hernandez M，Hernandez I，Rodriguez F，et al.Endothe-lial dysfunction in gestational hypertension induced bycatechol-O-methyltransfense inhibition[J].Exp Physiol，2013，98（3）：856-866.
|
| [8] |
Markandu ND，He FJ，Papavasileiou MV，et al.Urinarycatecholamines and the relationship with blood pressureand pharmacological therapy[J].J Hypertens，2016，34（4）：704-709.
|
| [9] |
李萍，关慧敏.早发型重度子痫前期患者终止妊娠孕周对母婴结局的影响研究[J].中国妇幼保健，2014，29（36）：6011-6012.
|
| [10] |
Lisonkova S，Joseph KS.Incidence of preeclampsia：risk factors and outcomes associated with early-versus late-onset disease[J].Am J Obstet Gynecol，2013，209 （6）：544 （e1-e12）.
|
| [11] |
杨桂玲.早发型重度子痫 前期终止妊娠时机对母儿结局的影响[J].中国伤残医学，2013，23（11）：156-157.
|
| [12] |
Salomon C，Kobayashi M，Ashman K，et al.Hypoxia-induced changes in the bioactivity of cytotrophoblast-derived exosomes[J].PLoS One，2013，8（11）：e79636.
|
| [13] |
黄瑾姝.早发型重度子痫前期终止妊娠时机与分娩方式对围生儿结局的影响分析[J].中国卫生产业，2014，11（12）：7-8，10.
|
| [14] |
曾瑞香，陈锦欢.早发型重度子（广间）前期终止妊娠时机与分娩方式对围生儿结局的影响[J].中国药物经济学，2015，10（6）：112-114.
|
| [15] |
Meng XF，Qiu SQ.The effect of termination of pregnancy on the outcome of pregnancy in early onset severe preeclampsia[J].Chin Contemp Med，2014，21（6）：52-53.
|
|
|
|
