齐齐哈尔地区脑卒中患者生物样本库的建立和质量鉴定

Abstract
Figure/Table
References (20)
Related Citation (15)

Download: PDF (0 KB) HTML (48 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To targeted collect the biological samples of patients with cerebral apoplexy in Qiqihar，to construct a standardized biological sample bank of cerebral apoplexy with high quality and provide sample sources and data supporting platform for the data analysis and clinical decisions for cerebral apoplexy in Qiqihar.Methods The standardized operative process was made，the object was selected，blood serum，blood plasma，white cells，DNA and RNA extracted from the white cells of the standardized patients were collected and stored in the refrigerator at-80℃or liquid nitrogen.The quality of DNA and RNA was detected so that clinical sample sources with high quality were to be provided.Results From 2013，a total of 753 patients were enrolled in the sample bank.Samples of blood serum and blood plasma were 3008 respectively，white cells were 2253 and DNA and RNA were 3770 respectively.The results of electrophoresis showed the RNA electrophoresis had both 18S and 28S stripes without dispersion and the lumen of 28S stripe was as twice as that of the 18S stripe.DNA electrophoresis had clear stripes with good integrity.Conclusion The standardized biological sample banks of cerebral apoplexy with high quality in Qiqihar is primarily established to further explore and apply the data sources of cerebral apoplexy，which can provide help for individualized treatment of patients with cerebral apoplexy in the future.

Key words： Cerebral apoplexy Biological sample bank Qiqihar

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WEI Dong-mei
	YIN Gang
	LIU Rui
	WANG Qiang
	WANG Chao-nan
	QIU Xue-rong

Cite this article:

WEI Dong-mei,YIN Gang,LIU Rui, et al. Establishment and quality detection of biological sample bank of cerebral apoplexy in Qiqihar[J]. 中国当代医药, 2016, 23(16): 8-11.

URL:

http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2016/V23/I16/8

［1］	Bonita R，Mendis S，Truelsen T，et al.The global stroke initiative［J］.Lancet Neurol，2004，3（7）：391-393.
［2］	陈竺.全国第三次死因回顾抽样调查报告［M］.北京：中国协和医科大学出版社，2008：1-5.
［3］	邱祝，向廷秀，任国胜.肿瘤生物样本库的标准化建立与管理［J］.重庆医学，2014，43（26）：3546-3547.
［4］	焦保华，梁朝辉，王苑宇，等.人脑胶质瘤标本库的初步建立与管理［J］.河北医科大学学报，2013，34（4）：388-390.
［5］	AibaidulaA，LuJF，WuJS，etal.Establishment and maintenance of a standardized glioma tissue bank：huashan experience［J］. Cell Tissue Bank，2015，16（2）：271-281.
［6］	邱学荣，于丽君，魏冬梅，等.齐齐哈尔地区缺血性脑卒中高危人群危险因素调查与研究［J］.理论观察，2015，4（2）：126-127.
［8］	陆怡.转化医学与生物样本库现状［J］.生命的化学，2012，32 （3）：287-293.
［9］	陈志琴，李华，张丽丽，等.新疆地区脑卒中数据库7250例临床分析［J］.中风与神经疾病杂志，2012，29（5）：454-456.
［10］	王炜，毕家香，周利民，等.湖南湘潭地区脑卒中数据库的初步建设及分析［J］.湖南师范大学学报（医学版），2013，10（4）：1-4.
［11］	袁芳.基于基因功能信息预测疾病相关基因［D］.武汉：华中科技大学，2008.
［7］	Meir K，Gaffney EF，Simenon-Dubach D，et al.The human face of biobank networks for translational research［J］.Biopreserv Biobank，2011，9（3）：279-285.
［12］	Watrin E，Kaiser FJ，Wendt KS.Gene regulation and chromatin organization：relevance of cohesin mutations to human disease［J］.Curr Opin Genet Dev，2016，37（3）：59-66.
［13］	袁力赟.复杂疾病的核心基因筛选及分子机制研究［D］.上海：上海交通大学，2012.
［14］	张育军，叶磊，高芳芳，等.生物样本库建设是临床医学研究的基石［J］.转化医学杂志，2014，3（6）：347-351.
［15］	Le Page C，Kobel M，de Ladurantaye M，et al.Specimen quality evaluation in Canadian biobanks participating in the COEUR repository［J］.Biopreserv Biobank，2013，11（2）：83-93.
［16］	赵晓航，钱阳明.生物样本库-个体化医学的基础［J］.转化医学杂志，2014，3（2）：69-73，83.
［17］	王聪.大肠癌组织库建立及质量控制的经验［D］.银川：宁夏医科大学，2012.
［18］	姚静怡，杨爱婷，李秀红.样本库质量控制评估体系的建立［J］.临床和实验医学杂志，14（21）：1758-1760.
［19］	Yu K，Zhang J，Li X，et al.Establishment and management of a lung cancer biobank in Eastern China［J］.Thorac Cancer，2015，6（1）：58-63.
［20］	Yu C，Lyu J，Chen Y，et al.Epidemiology of major depressive episodes among Chinese adμlts aged 30-79 years：data from the China Kadoorie Biobank［J］.Zhonghua Liu Xing Bing Xue Za Zhi，2015，36（1）：52-56.