骨髓间充质干细胞对小鼠肺脏缺血再灌注损伤的影响

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Abstract ObjectiveTo investigate the influence of bone marrow mesenchymal stem cells(BMSCs)on lung ischemiareperfusion injury(LIRI)in mice.MethodsBMSCs from rats were isolated,cultured and purified by the whole bone marrow acherence method.LIRI was produced by occlusion of the left hilum of lungs for 60 min followed by 240 min reperfusion.70 mice were randomly divided into 7 groups(n=10),included the sham operation group(the Sham group),the ischemia-reperfusion group(the I/R group),the BMSCs 1 group(tail vein injections of 1×106BMSCs),the BMSCs 2 group(tail vein injections of 2×106BMSCs),the BMSCs 5 group(tail vein injection of 5×106BMSCs),the BMSCs 10 group(tail vein injection of 10×106BMSCs),the BMSCs it group(endotracheal injection of 5×106BMSCs).At the end of reperfusion,blood samples were obtained for blood gas analysis and PaO2/FiO2was calculated.Then,the animals were sacrificed and lung tissues were immediately removed for determination of superoxide dismutase(SOD),tumor necrosis factor-α(TNF-α),interleukin-8 (IL-8),and myeloperoxidase (MPO)and for microscopic examination of the pathological changes of lungs which were scored.ResultsCompared with Sham group,PaO2/FiO2was significantly decreased,the pathological scores and the levels of TNF-α,IL-8,and MPO were increased,and SOD activity was decreased in I/R,BMSCs 1,BMSCs 2,BMSCs 5,BMSCs 10,BMSCs it group,the difference was statistically significant (P＜0.05).Compared with I/R group,PaO2/FiO2was significantly increased,the pathological scores and the levels of TNF-α,IL-8,and MPO in lung tissues were decreased,and SOD activity was increased in BMSCs 1,BMSCs 2,BMSCs 5,BMSCs 10,BMSCs it group,the difference was statistically significant(P＜0.05).The most obvious group is BMSCs 10,the difference was statistically significant(P＜0.05).There is no obvious difference between endotracheal injection and intravascular injection with the same concentration of BMSCs,the difference was not statistically significant(P＞0.05).ConclusionBMCS can alleviate LIRI in mice,and the responsible mechanism is related to inhibition of inflammatory responses.The lung protective effect is dose dependent.There is no significant difference between endotracheal injection and intravascular injection with the same concentration of BMSCs.

Key words： Bone marrow mesenchymal stem cells Lung Reperfusion injury Oxygen free radical

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	Articles by authors
	SUN Chang-rong
	HOU Nian-guo
	LI Hui
	SHUAI Xun-jun
	SUN Ming-jie
	TANG Yu-ru
	XU Tang-wen
	AI Deng-bin

Cite this article:

SUN Chang-rong,HOU Nian-guo,LI Hui, et al. Influence of bone marrow mesenchymal stem cells on lung ischemiareperfusion injury in mice[J]. 中国当代医药, 2018, 25(5): 18-21.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2018/V25/I5/18

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