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| Effect and mechanism of Com pound C on the neurotoxic injury of Bupivacaine |
| LU Jun1 JU You-ting2 HU Yan-hui1 CHEN Shou-lin1 CAIJun-ying1 LIChang1 |
1.Department of Anesthesiology,the Second Affiliated Hospital of Nanchang University,Jiangxi Province,Nanchang 330006,China;
2.Department of Anesthesiology,Affiliated Stomatological Hospital of Nanchang University,Jiangxi Province,Nanchang 330006,China |
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Abstract Objective To investigate the effect of Compound C on neurotoxicity injury of Bupivacaine and mechanism. M ethods Eighteen normalmale SD ratswere divided into 3 groups randomly,6 rats in each group.Normal group(group N):the rats were received intrathecal injection with normal 30μl and intraperitoneal injection with normal saline;2％ Bupivacaine group(group B):the ratswere received intrathecal injection with 2％Bupivacaine 30μl and intraperitoneal injection with normal saline.2％Bupivacaine combined with Compound C group(group B＋C):the ratswere received intrathecal injection with 2％Bupivacaine 30μl combined with intraperitoneal injection with 20 mg/kg Compound C.The thresholds ofmechanical pain were measured by using Von-Frey hair at 2 h,3 h,4 h,1 d,and 2 d after treatment,and motor function(MF)score was performed.The ratswere sacrificed 3 d after treatment and the expression of AMPK in the dorsal root ganglia of rats was examined by fluorescent quantitation PCR.Results 30 min after intrathecal injection with Bupivacaine,compared with the group N,the MF scores of the group B and group B＋C were increased obviously (P＜0.05),and the MF scores of the group B and group B＋C were recovered 4 h after treatment.1 d After intrathecal in jection with Bupivacaine,the thresholds ofmechanical pain decreased in group B,compared with those of group B＋C and group N(P＜0.05).The fluorescent quantitation PCR results indicated that,the expressions of AMPK in group B＋C was lower than that of the group B and group N(P＜0.05).Conclusion Compound C can attenuate the neurotoxicity injury of Bupivacaine,and themechanism may be that it inhibited the expression of AMPK and protected the neural cells.
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