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| The effects of Thalidomide on the proliferation,IL-6 and Cx43 expression of prostate cancer PC3 cells |
| YIN Wei-hua1 XIA Hong-mei1 LUO Xiao-jin2▲ XU Rong1 HU Xiao-xiong1 |
1.Department of Oncology,People′s Hospital of Yichun City in Jiangxi Province/Clinic Medicine School of Yichun university,Yichun 336000,China;
2.Department of Urinary Surgery,People′s Hospital of Yichun City in Jiangxi Province/ Clinic Medicine School of Yichun university,Yichun 336000,China |
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Abstract Objective To observe the effects of Thalidomide on the proliferation and apoptosis of prostate cancer PC3 cells,as well as on the expression of the protein Cx43 and IL-6 in this cells.Methods Thalidomide with different concentrations (6.25,12.50,25.00,50.00,100.00 μg/ml)was added to PC3 cells at the logarithmic growth phase.MTT was used to detect the inhibitory effect of different concentration of Thalidomide on proliferation of PC3 cells,the apoptosis rate of PC3 cells was studied by Annexin V fluorescein isothiocyanate（Annexin PI）double staining with flow cytometry.Enzyme-linked Immunosorbnent assay method was used to detect the expression of IL-6 and Cx43 in PC3 cells. Results Below 25.00,50.00,100.00 μg/ml,Talidomide significantly inhibited the proliferation of PC3 cells in vitro,and the inhibition rate increased with the increase of time and concentration,the expression of IL-6 decreased with the increasing of concentration(r=-0.924，P=0.009),the expression of Cx43 had positive correlation with the concentration of thalidomide(r=0.915，P=0.010).Conclusion Thalidomide can inhibit the expression of IL-6 in PC3 cells,increase the expression levels of Cx43,restore the functional refreshment of gap junction intercellular communication function in prostate cancer PC3 cells for in inhibiting the proliferation of PC3 cells and inducing its apoptosis,in order to inhibit the growth of tumor.
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