Experimental study on the effect of Silybin on skin quality of nude mice
WANG Xue1 FU Wei2 YANG Liu1 LI Jian-min1▲ XU Yan-ming1▲
1.Experimental Center,Jiamusi College of Heilongjiang University of Traditional Chinese Medicine,Jiamusi 150047,China;
2.Department of Medicine,Cancer Hospital of Jiamusi City in Heilongjiang Province,Jiamusi154007,China
Abstract：ObjectiveTo study the influence of Silybin cream on skin damage of nude mice model induced by UVB,including skin moisture,pH value,melanin and heme.Methods40 nude mice were divided into 4 group,they were the blank group,the model group,the positive control group and the Silybin group.The blank group was given a touch every day,and the model group was given UVB exposure every day after touching.Nude mice′s back in the positive control group and the Silybin group was smeared by Shiseido yurao zhenlai and Silybin cream before irradiated by UVB,nude mice in the model group,the positive control group and the silybin group were irradiated by UVB for 15 days continually,each time 20 min,the back of nude mice was damaged,the back was tested by the skin tester produced by German CK company after irradiating.ResultsThe moisture content in the model group decreased distinctively compared with the blank group,pH value increased,melanin and heme content increased,with significant difference(P＜0.05).The moisture content in the positive control group and the Silybin group increased compared with the blank group and the model group,pH value decreased,melanin and heme content decreased,with significant difference(P＜0.05).ConclusionSilybin can increase skin moisture,decrease hyper pigmentation,reduce skin damaged by outside stimulus,and repair damaged skin effectively.
王雪;付巍;杨柳;李建民;徐艳明. 水飞蓟宾对裸鼠皮肤质量影响的实验研究[J]. 中国当代医药, 2017, 24(30): 117-119.
WANG Xue;FU Wei;YANG Liu;LI Jian-min; XU Yan-ming. Experimental study on the effect of Silybin on skin quality of nude mice. 中国当代医药, 2017, 24(30): 117-119.
Kim MK，Choi SY，Byun HJ，et al.Comparison of sebum secretion，skin type，pH in humans with and without acne[J].Arch Dermatol Res，2006，298（3）：113-119
[6]
杨柳，邓燕，赖梅生，等.中医皮肤美容学[M]北京：人民军医出版社，2011：99.
[7]
Runeman B，Faergemann J，Larko O.Experimental candida albicans lesions in healthy humans：dependence on skin pH[J].Acta Derm Venereol，2000，80（6）：421-424.
[8]
BehneMJ，BarryNP，HansonKM，et al.Neonatal development of the stratum corneum pH gradient：localization and mechanisms leading to emergence of optimal barrier function[J].J Invest Dermatol，2003，120（6）：998-1006.
[9]
FluhrJW，BehneMJ，BrownBE，et al.Stra-tum corneum acidification inneonatal skin：secretory phospholip ase A2 and the sodium/hydrogenantiporter-1 acidify neonatal rat stratum corneum[J].J Invest Dermatol，2004，122（2）：320-329.
[10]
Behne MJ，Meyer JW，Hanson KM，et al.NHE 1 regulates the stratum corneum permeability barrier homeostasis.Microenvironment acidification assessed with fluorescence lifetimeimaging[J].JBiolChem，2002，277（49）：47399-47406.
[11]
Hachem JP，Behne MJ，AronchikI，et al.Extracellular pH controls NHE 1 expression in epidermis and keratinocytes：implications for barrier repair[J].J Invest Dermatol，2005，125（4）：790-797.
Clarys P，Alewaeters K，Lambrecht R，et al.Skin color measurements：comparison between three instruments：the Chromameter，the DermaSpectrometer and the Mexameter?[J].Skin Res Technol，2000，6（4）：230-238.
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