干式融浆机和水浴式融浆机溶解冷沉淀及溶解时间的长短对冷沉淀中凝血因子生物活性的影响

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摘要目的探讨使用干式和水浴式融浆机两种方式融解冷沉淀以及融解时间长短对冷沉淀中凝血因子生物活性的影响,为达到更好的融浆效果提供依据。方法选取2016年2月3~7日我院宝安中心血站提供的120袋冷沉淀（规格1.0 U/袋）,采集时间相近,均不能超过5 d,将120袋冷沉淀随机分为两组,每组60袋,编为1组和2组。1组采用干式融浆机溶解,2组采用水浴式融浆机溶解,对120份置于不同融浆机37℃融解10、20、30 min的冷沉淀样品,分别测定FⅧ、VWF抗原、PT、TT、APTT及Fib。结果两组的融浆效果及各个检测指标比较,差异无统计学意义（P>0.05）。但随着融浆时间的延长,会导致APTT延长,FⅧ、VWF抗原活性下降,差异有统计学意义（P<0.05）。结论融浆时间延长会导致凝血因子FⅧ、VWF抗原活性下降,APTT延长,为达到更好的临床输血疗效,推荐融解冷沉淀的最佳时间为10 min。

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	杨庆文
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关键词 ：不同融浆方式, 冷沉淀凝, 血因子, 融浆时间

Abstract：Objective To investigate the use of dry and water-bath blood soluble machine for cryoprecipitation dissolution and the effect of length of dissolution time on the biological activity of coagulation factors in cryoprecipitate,in order to provide the basis for better results.Methods Altogether 120 bags of cryoprecipitate (Specification:1.0 U/Bag) with similar blood collection dates (less than 5 days) that were provided by our Central Blood Station of Baoan from February 3^rd to February 7^th in 2016 were selected and randomly divided into two groups (group 1 and group 2),with 60 bags in each group.group 1 adopted dry blood soluble machine for cryoprecipitation dissolution;while,group 2 adopted water-bath blood soluble machine for cryoprecipitation dissolution.FactorⅧ (FⅧ),von Willebrand factor antigen (vWF-Ag),prothrombin time (PT),thrombin time (TT),activated partial thromboplastin time (APTT) and plasma fibrinogen (Fib) of 120 cryoprecipitate samples that were placed in different blood soluble machines at 37℃ for 10,20 min and 30 min were determined.Results There was no statistically significant difference between group 1 and group 2 in the effects of blood plasma dissolution and all the indexes (P>0.05);however,with the prolonging of dissolution time,prolonged APTT as well as decreased activity of FⅧ and VWF antigen were seen,with statistically significant difference (P<0.05).Conclusion Prolonged blood plasma dissolution time will lead to decreased activity of FⅧ and VWF antigen as well as prolonged APTT,with statistically significant difference.To achieve better efficacy of clinical blood transfusion,the best time for cryoprecipitation dissolution is recommended to be 10 min.

Key words： Different blood plasma dissolution methods Cryoprecipitation Coagulation factor Dissolution time

收稿日期: 2017-06-15

PACS:

R457.1

引用本文:

杨庆文, 李广莹, 丁文. 干式融浆机和水浴式融浆机溶解冷沉淀及溶解时间的长短对冷沉淀中凝血因子生物活性的影响[J]. 中国当代医药, 2017, 24(24): 118-120.
YANG Qing-wen, LI Guang-ying, DING Wen. The effect of cryoprecipitation dissolution with dry blood soluble machine and water-bath blood soluble machine and the length of dissolution time on the biological activity of coagulation factor in cryoprecipitate. 中国当代医药, 2017, 24(24): 118-120.

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http://dangdaiyiyao.com/CN/ 或 http://dangdaiyiyao.com/CN/Y2017/V24/I24/118

[1]	梁其隆,梁若鹄,陈龙菊,等.冷沉淀解冻后凝血因子变化的实验研究[J].中国医药导报,2012,9（11）：28-30.
[2]	邹宗丽,甘芳香,车火娇,等.冷沉淀在不同温度不同时间放置对Ⅷ因子的影响[J].医学检验,2012,19（36）：90-91.
[3]	范红玲.单采血小板联合冷沉淀在大出血患者临床治疗中的应用[J].中国实用医刊,2012,39（7）：105-106.
[4]	陈善华,朱丽莉,吕红艳,等.保证临床用血的应对措施探析[J].中国输血杂志,2011,24（8）：695-696.
[5]	何子毅,田兆嵩.冷沉淀的临床应用[J].中国输血杂志,2008,21（12）：986.
[6]	Neimark AI,Gatkin ML.Use of cryoprecipitate in combined therapy of acute purulent pyelonephritis[J].Urologiia,2005, 4：42-48.
[7]	刘景汉,王德清.临床输血学[M].北京：人民卫生出版社,2011：40.
[8]	苏红,谭爱玲,徐少英,等.冷沉淀制备的关键控制环节[J].中国医学创新,2012,10（10）：144-145.
[9]	万鹏,童华生,张兴钦,等.新型凝血和血小板功能分析仪在弥散性血管内凝血早期诊断中的价值[J].中国急救医学,2014,10（9）：789-793.
[10]	胡豫.2012版弥散性血管内凝血诊断与治疗中国专家共识解读[J].临床血液学杂志,2013,2（11）：978-979.
[11]	Ettinger A,Miklauz MM,Bihm DJ, et al .Preparation of cryoprecipitate from riboflavin and UV light-treated plasma[J].Transfus Apher Sci,2012,6（46）：153-158.
[12]	Funk MB,Heiden M,Volkers P, et al .Evaluation of risk min-imisation measures for blood components-based on reporting rates of transfusion-transmitted reactions （1997-2013）[J].Transfus Med Hemother,2015,42（4）：240-246.
[13]	陈志军,吴稷,樊琴红,等.冷沉淀制备技术在血液成分制备中的应用[J].中国中医药咨讯,2011,3（11）：69-70.
[14]	Bui E,Inaba K,Ebadat A, et al .The impact of increased plasmaratios in massively transfused trauma patients：a prospective analysis[J].Eur J Trauma Emerg Surg,2016,42（4）：519-525.
[15]	Shaz BH,Stowell SR,Hillyer CD.Transfusion-related acute lung injury：from bedside to bench and back[J].Blood,2011, 117（5）：1463-1471.