射频能量对关节软骨细胞活性的实验研究

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Abstract Objective To study the effect of radiofrequency energy on the cell activity of articular cartilage,so as to verify the clinical feasibility of this technique.Methods A total of 10 pairs of bovine articular cartilage were selected,of which five pairs were used as radiofrequency energy subjects.The sides of each pair were divided into four table cells,of which three ones were for research,respectively corresponding to SCULPTOR monopolar radiofrequency head,SAPHYRE bipolar radiofrequency head,and TAC-CⅡcartilage temperature-controlled head for 1 min.The remaining one was as a control without radiofrequency.The rest five pairs were selected as experimental subjects in different processing time(5,10,20,and 30 second),of which four pairs disposed with SAPHYRE bipolar radiofrequency head were selected as the research group and the last one without disposal was selected as the control group.The above-mentioned research groups and control groups were compared in order to observe the differences of hematoxylineosin staining(HE staining),fluorescence staining,and GAG release rate,and the differences of cell activity and tissue damage were analyzed.Results The vacuolization rate and mortality rate of the HE staining and fluorescence staining in the SCULPTOR monopolar radiofrequency head group,the SAPHYRE bipolar radiofrequency head group,and the TAC-CⅡcartilage temperature-controlled head group were significantly higher than those of the control group,and the differences were statistically significant(P<0.05).On the first day,the soft tissue GAG release rate in the SCULPTOR monopolar radiofrequency head group,the SAPHYRE bipolar radiofrequency head group,and the TAC-CⅡcartilage temperature-controlled head group was significantly lower than that in the control group;on the second and third days,the GAG release rate of soft tissue of the SAPHYRE bipolar radiofrequency head group and the TAC-CⅡcartilage temperature-controlled head group was significantly lower than that of the control group,and the differences were statistically significant(P<0.05).The vacuolization rate and the mortality rate of the HE staining and fluorescence staining in the 5,10,20,and 30 s treatment groups were significantly higher than those in the control group,and the differences were statistically significant(P<0.05).On the first,second,and third days,the GAG release rate of soft tissue in the 5,10,20,and 30 s treatment groups were significantly lower than that in the control group,and the differences were statistically significant(P<0.05).Conclusion The increase of radiofrequency energy and processing time both will lead to the damage of the joint soft tissue in a positive correlation.Therefore,it is necessary to appropriately reduce the radiofrequency energy and processing time in order to avoid tissue damage in patients during treatment.

Key words： Radiofrequency energy Articular cartilage Cellular activity Hematoxylin-eosin staining GAG release rate

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	XIAO Jian-hua

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XIAO Jian-hua. Experimental study of radiofrequency energy on articular chondrocyte activity[J]. 中国当代医药, 2018, 25(21): 120-123.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2018/V25/I21/120

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