Preparation of coenzyme Q10 liposome and its anti-ultraviolet damage effect
WU Hai-you1,2 WU Yi2 WU Tie2,3▲
1.Dongguan Food and Drug Inspection Institute,Guangdong Province,Dongguan 523808,China;
2.Guangdong Medical University,Guangdong Province,Zhanjiang 524023,China;
3.Coenzyme Q10 Joint Research Center,Guangdong Medical University-Guangdong Runhe Biotechnology Co.,Ltd.,Guangdong Province,Dongguan 523808,China
Abstract：Objective To study the preparation of coenzyme Q10 liposome and its anti-ultraviolet damage effect.Methods The coenzyme Q10 liposomes were prepared by injecting ethanol,then animal experiments were performed.A total of 32 SPFmice were randomly divided into normal(CON)group,model(MOL)group,coenzyme Q10 liposome(COQ)group,and titanium dioxide(TiO2)group,8mice in each group.Themice in the CON group were not subjected to any treatment after depilation,and the remaining groups ofmice were smeared with the corresponding cream and irradiated with ultraviolet rays every day after depilation.Among them,the MOL group was smeared with the blank cream,the COQ group was smeared with the coenzyme Q10 liposome,and the TiO2 group was smeared with the TiO2 cream.The irradiation dose was theminimum amount of erythema,and the experiment lasted 8 weeks.After the end of the experiment,the skin pathology and the contents of malondialdehyde(MDA),superoxide dismutase(SOD),and matrix metalloproteinase(MMP-1),glutathione peroxidase(GSH-Px)in themice were determined.Results The optimum formulation of coenzyme Q10 liposome showed as the temperature was 60°C,the water phase of pH was 7.7 and other was 0.9%of coenzyme Q10 and 1.6%of lecithin.The encapsulation efficiency of coenzyme Q10 liposome was 44.7%,and the liposome was uniform in size and globular.The MDA content and the relative expression of MMP-1 in skin tissue of the mice in the MOL group were higher than those in the CON group,and the SOD contentwas lower than that in the CON group,with statistically significant differences(P＜0.05).The SOD content in the skin tissue of themice in the COQ group was higher than that in the MOL group,and the relative expression of MMP-1 was lower than that in the MOL group,the differences were statistically significant(P＜0.05).The MDA content in the skin tissue of themice in the TiO2 group was lower than that in the MOL group,and the difference was statistically significant(P＜0.05).The GSH-Px content in the skin tissue ofmice in the COQ group was higher than that in other groups,and the differences were statistically significant(P＜0.05).Conclusion The prepared coenzyme Q10 liposome is uniform in size and has anti-ultraviolet damage effect onmice skin.
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