咖啡酸对2型糖尿病大鼠血糖浓度的影响

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摘要目的探讨咖啡酸对2型糖尿病大鼠血糖浓度的影响。方法选取60只6～8周龄雄性SD大鼠，适应性饲养3 d后，随机取20只大鼠作为正常观察组，分为空白组和实验组1～3，每组5只。实验组1～3分别以0.4、0.6、0.8 g/kg咖啡酸灌胃，空白组以1 ml/100 g等量蒸馏水灌胃。其余40只大鼠高糖高脂饲料连续喂养6周，连续3 d腹腔内注射30 mg/kg的链脲佐菌素（STZ），用于2型糖尿病大鼠模型的建立，FPG>7 mmol/L及大鼠尿糖实验检测阳性为造模成功。将造模成功的30只大鼠随机分为模型组、阿卡波糖组、联合组及咖啡酸组1～3，每组5只。阿卡波糖组予以10 mg/kg的阿卡波糖灌胃，联合组采用10 mg/kg的阿卡波糖以及1 g/kg的咖啡酸联合灌胃，咖啡酸组1～3分别采用0.8、1.0、1.2 g/kg咖啡酸灌胃，模型组以1 ml/100 g等量蒸馏水灌胃。观察不同时间咖啡酸对正常观察组大鼠空腹血糖浓度（FPG）及胰岛素（INS）的影响；观察各药物组对2型糖尿病大鼠FPG浓度的影响。结果灌胃前，灌胃第1、6天，各组（正常观察组中）大鼠的FPG浓度比较，差异均无统计学意义（P＞0.05）。灌胃前，各组（模型组和各药物组）的FPG浓度比较，差异均无统计学意义（P＞0.05）；灌胃后，与模型组比较，各药物组大鼠的FPG浓度均显著降低，差异有统计学意义（P＜0.05，P＜0.01），联合组的FPG浓度降低最明显，差异有统计学意义（P＜0.01）；各药物组中，以联合组的降糖效果最明显（P＜0.05）。结论咖啡酸对2型糖尿病大鼠具有降FPG作用，但不如阿卡波糖的降糖效果显著。阿卡波糖联合咖啡酸的降糖效果最明显。

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	裴瑜
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	何爱华
	阳昊
	周鸿
	邹建中
	李桦楠

关键词 ：咖啡酸, 阿卡波糖, 2型糖尿病, 降血糖

Abstract：Objective To explore the influence of Caffeic Acid on blood glucose concentration in rats with type 2 diabetes mellitus.Methods Sixty male SD rats aged 6-8 weeks were selected and fed for 3 days.Twenty rats were selected as the normal observation group and they were divided into the blank group and experimental group1-3,and there were 5 rats in each group.The experimental group 1～3 was fed with 0.4,0.6 and 0.8 g/kg of Caffeic Acid respectively,the blank group was gavaged with 1 ml/100 g of equivalent distilled water.The other 40 rats were fed with high-sugar and high-fat diet for 6 weeks and intraperitoneally injected with 30 mg/kg Streptozotocin(STZ)for 3 days,the model of type 2 diabetic rats was established,FPG>7 mmol/L and rat urine sugar test positive were considered to be successful in modeling.Thirty rats successfully modeled were randomly divided into model group,Acarbose group,combined group and Caffeic Acid group 1-3,and there were 5 rats in each group.Acarbose group was given 10 mg/kg Acarbose,combined group was given 10 mg/kg of Acarbose and 1 g/kg of Caffeic Acid,Caffeic Acid group 1-3 was given 0.8,1.0,1.2 g/kg respectively,model group was given 1 ml/100 g of distilled water.The influence of Caffeic Acid on fasting blood glucose(FPG)and insulin(INS)in rats of normal observation group was observed at different times;the influence of each drug group on the concentration of FPG in type 2 diabetic rats was observed.Results There was no significant difference in FPG concentration and INS expression level among the groups (in normal observation group)before gavage,gavage for the first,sixth day(P>0.05).There was no significant difference in FPG concentration among the groups(in model group and various drug group)before gavage(P>0.05).After gavage,compared with model group,the FPG concentration in various drug group were decreased significantly,and the differences were statistically significant(P＜0.05,P＜0.01).The FPG concentration in combined group was significantly lower,and the difference was statistically significant(P＜0.01).Among the drug groups,the combined group had the most significant hypoglycemic effect(P＜0.05).Conclusion Caffeic Acid has a reducing FPG effect on type 2 diabetic rats,but it is not as effective as Acarbose in reducing blood sugar.The effect of Acarbose combined with Caffeic Acid was the most obvious.

Key words： Caffeic Acid Acarbose Type 2 diabetes mellitus Reducing the blood glucose

基金资助:重庆市基础科学与前沿技术研究专项一般项目（cstc2015jcyjA50001）

引用本文:

裴瑜;董旭;唐心一;何爱华;阳昊;周鸿;邹建中;李桦楠. 咖啡酸对2型糖尿病大鼠血糖浓度的影响[J]. 中国当代医药, 2018, 25(25): 16-18转22.
PEI Yu;DONG Xu;TANG Xin-yi;HE Ai-hua;YANG Hao;ZHOU Hong;ZHOU Jian-zhong;LI Hua-nan. Influence of Caffeic Acid on blood glucose concentration in rats with type 2 diabetes mellitus. 中国当代医药, 2018, 25(25): 16-18转22.

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