间质性肺疾病患者呼出气冷凝液中8-异前列腺素及白三烯B4的水平及临床意义

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摘要目的探讨间质性肺疾病（ILD）患者呼出气冷凝液（EBC）中8-异前列腺素（8-iso-PG）及白三烯B4（LTB4）的水平及其临床意义。方法选取2012年8月～2013年12月在我科住院的20例ILD患者为ILD组，20例年龄、性别、体重指数等均相匹配的健康体检者为对照组，采用ELISA检测EBC中8-iso-PG及LTB4水平。结果ILD组EBC中8-iso-PG水平为（9.69±2.8）ng/L，高于对照组的（4.86±1.9）ng/L，差异有统计学意义（P＜0.05）；ILD组EBC中LTB4水平为（15.46±6.6）ng/L，高于对照组的（8.55±4.4）ng/L，差异有统计学意义（P＜0.01）。8-iso-PG、LTB4的特异度均为85%，敏感度分别为90%、60%。结论8-iso-PG及LTB4在ILD患者EBC中升高，测定EBC生物标志物或可用于评价ILD炎症反应。

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	黄立霞
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	巫翠华
	洪永忠
	钟春
	朱一鑫

关键词 ：间质性肺疾病, 呼出气冷凝液, 8-异前列腺素, 白三烯B4

Abstract：Objective To investigate the levels of 8-isoprostane(8-iso-PG) and leukotriene B4(LTB4) in exhaled breath condensate(EBC)and its clinical significance in interstitial lung disease(ILD).Methods 20 ILD patients in our department from August 2012 to December 2013 were selected as the ILD group，20 healthy subjects matched with age and body mass index were selected as control group.The levels of 8-iso-PG and LTB4 in EBC were measured by ELISA.Results The level of 8-iso-PG in ILD group was (9.69+2.8) ng/L，which was higher than that in control group(4.86+1.9) ng/L，with significant difference (P＜0.05).The level of LTB4 in EBC of ILD group was (15.46+6.6) ng/L，higher than that in control group (8.55+4.4) ng/L，the difference was statistically significant(P＜0.01).The specificity of LTB4 and 8-iso-PG was 85% respectively，and the sensitivity was 90% and 60% respectively.Conclusion 8-iso-PG and LTB4 increases in EBC of ILD patients.Measuring biomarkers in EBC may be useful to evaluate inflammatory response of ILD.

Key words： Interstitial lung disease Exhaled breath condensate 8-isoprostane Leukotriene B4

作者简介: 广东省深圳市科技计划项目（201203208）

引用本文:

黄立霞;李小莉;黄晓星;巫翠华;洪永忠;钟春;朱一鑫. 间质性肺疾病患者呼出气冷凝液中8-异前列腺素及白三烯B4的水平及临床意义[J]. 中国当代医药, 2016, 23(3): 34-36.
HUANG Li-xia;LI Xiao-li;HUANG Xiao-xing;WU Cui-hua;HONG Yong-zhong;ZHONG Chun;ZHU Yi-xin. Levels of 8-isoprostane and leukotriene B4 in exhaled breath condensate and its clinical significance in interstitial lung disease. 中国当代医药, 2016, 23(3): 34-36.

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