Influence of PM2.5 on liPid metabolism and oxidative stress of hePatocyte
LI Ming1 XIE Jing-jing1 WU Tong2 WANG Ying1 LIU Fang-fang2 WANG Fang1 LI Yan2▲
1.Schoo1 of Basic Courses,Guangdong Pharmaceutica1 University,Guangzhou 510006,China;
2.Co11ege of Basic Medica1 Sciences,Guangzhou University of Traditiona1 Chinese Medicine,Guangzhou 510006,China
Abstract：Objective To exp1ore the inf1uence of PM2.5 on 1ipid metabo1ism and oxidative stress of hepatocyte. Methods PM2.5 was co11ected from atmosphere in urban area of Guangzhou in 2014.Toxicity of 1iver L02 ce11 was ana1yzed by CCK-8 at PM2.5 1eve1s of 6.25-100 μg/m1.The group of 6.25 μg/m1,12.5 μg/m1,25 μg/m1 was set respective1y,and the negative contro1 group was set.The contents of intrace11u1ar trig1yceride and cho1estero1,the expression 1eve1 of LXRα and SREBP-1c,the intrace11u1ar oxygen free radica1 1eve1 among four groups was compared. Results When the PM2.5 concentration was 50 g/m1,which had obvious toxicity to 1iver L02 ce11s.The concentration of triacy1g1ycero1 in the 1iver ce11s in each dose group was higher than that in the negative contro1 group,with significant difference (P＜0.05). The expression 1eve1 of LXRα and SREBP-1c in the group of 25.00 g/m1 was higher than that in the negative contro1 group,with significant difference (P＜0.01).The 1eve1 of oxyradica1 in each dose group was higher than that in the negative contro1 group,with significant difference (P＜0.01). Conclusion PM2.5 can induce 1ipid accumu1ation in hepatocytes via LXRα and SREBP-1c,and induce oxidative stress in hepatocytes,which may be the important mechanism of PM2.5 causing non-a1coho1ic fatty 1iver disease.
Jia Q，Xia Y，Zhang Q，et al.Dietary patterns are associated with preva1ence of fatty 1iver disease in adu1ts[J].Eur J C1in Nutr，2015，69（8）：914-921.
[6]
Periasamy S，Chien SP，Chang PC，et al.Sesame oi1 mitigates nutritiona1 steatohepatitis via attenuation of oxidative stress and inf1ammation：a ta1e of two-hit hypothesis[J].J Nutr Biochem，2014，25（2）：232-240.
[7]
Karottki DG，Spi1ak M，Frederiksen M，et al.Indoor and outdoor exposure to u1trafine，fine and microbio1ogica11y derived particu1ate matter re1ated to cardiovascu1ar and respiratory effects in a pane1 of e1der1y urban citizens[J].Int J Environ Res Pub1ic Hea1th，2015，12（2）：1667-1686.
[8]
Carva1ho DF，Guimar˜aes MT，Ribeiro TS，et al.Preva1ence of 1iver diseases as referred by peop1e 1iving in the Santos and S˜ao Vicente Estuary[J].Environ Sci Po11ut Res Int，2015，22（19）：14579-14588.
[9]
Tong L，Li K，Zhou Q.The association between air po11utants and morbidity for diabetes and 1iver diseases modified by sexes，ages，and seasons in Tianjin，China[J].Environ Sci Po11ut Res Int，2015，22（2）：1215-1219.
[10]
Zheng Z，Xu X，Zhang X，et al.Exposure to ambient particu1ate matter induces a NASH-1ike phenotype and impairs hepatic g1ucose metabo1ism in an anima1 mode1[J].J Hepato1，2013，58（1）：148-154.
[11]
Vesterda1 LK，Danie1sen PH，Fo1kmann JK，et al.Accumu1ation of 1ipids and oxidative1y damaged DNA in hepatocytes exposed to partic1es[J].Toxico1 App1 Pharmaco1，2014，274（2）：350-360.
京公网安备 11010502046607号