1.School of Medical Technology,Qiqihar Medical University in Heilongjiang Province,Qiqihar 161006,China;
2.Immunology Teaching and Research Section,Qiqiharmedical University in Heilongjiang Province,Qiqihar 161006,China
Abstract：ObjectiveTo investigate the influence of freeze dried powder of Crassocephalum crepidioides on the expression of serum cytokines TNF-α,IL-6,IL-8 and IL-10 in ratswith ulcerative colitis(UC).Methods 76 healthymale SD rats were randomly selected.Twelve rats were randomly selected as blank control group.The other rats were induced with TNBS/ethanolmixture to establish UC ratmodel.After successfulmodel,all rats were treated with different drugs every day to observe the disease activity index(DAI).The level of TNF-α,IL-6,IL-8 and IL-10 in the serum weremeasured by ELISA.ResultsCompared with model group and positive control group,the level of proinflammatory cytokines IL-6,IL-8 and TNF-α were decreased in varying degrees(P＜0.01 or P＜0.05),and the level of anti-inflammatory factor IL-10 were increased in varying degrees in different dose group of Crassocephalum crepidioides (P＜0.01 or P＜0.05).ConclusionThe freeze-dried powder of Crassocephalum crepidioides has a certain effect on the symptoms of UC rats.Themechanism may be related to influencing the expression of TNF-α,IL-6,IL-8 and IL-10.
Marines LW，Fitzpatrick LR，French KJ，et al.Superpression of ulcerative colitis in mice by orally available inhibitors of sphingosine kinase[J].Dig Dis Sci，2008，53（4）：997.
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