Study on the mechanism of Liqi Buxue Decoction in delaying denervated skeletal muscle atrophy by regulating TGF-β1/Smad pathway
ZHAO Chang-yun-lei1 LIU Jun-ning2 ZHAO Yu1 GUO Shuang1 LI Nan2 NIU Su-sheng2 ZHANG Yan1▲
1.School of Traditional Chinese Medicine,Fujian University of Traditional Chinese Medicine,Fujian Province,Fuzhou 350122,China;
2.Key Laboratory of Orthopedics&Traumatology of Traditional Chinese Medicine and Rehabilitation Ministry of Education,Fujian University of Traditional Chinese Medicine,Fujian Province,Fuzhou 350122,China
Abstract：Objective To explore the effect and mechanism of Liqi Buxue Decoction on denervated skeletal muscle atrophy.Methods A total of 54 SD rats were randomly divided into the sham operation group (n=18),the model group(n=18)and the Liqi Buxue Decoction group (n=18).The gastrocnemius muscle atrophy model was made by cutting off the right tibial nerve.The wet weight ratio of gastrocnemius muscle was measured after 7,28 56 days,the cross-sectional area and diameter of muscle fibers and the volume of collagen fibers were measured by HE staining and Masson staining,the expression of TGF-β1,Smad2,Smad3,MyoD1 and MuRF1 mRNA in gastrocnemius muscle was detected by quantitative real-time PCR,and the expression of myosin was detected by immunofluorescence.Results After 7,28,56 days of the intervention,frum muscle wet weight ratio of the model group and the Liqi Buxue Decoction group was lower than that of the sham operation group in the same period,the difference was statistically significant(P＜0.05).After 7,28,56 days of the intervention,frum muscle wet weight ratio of the the Liqi Buxue Decoction group was higher than that of the model group in the same period,the difference was statistically significant(P＜0.05).After 7,28,56 days of the intervention,muscle fiber cross-sectional area and diameter of the model group and the Liqi Buxue Decoction group were lower than those of the sham operation group in the same period,the differences were statistically significant(P＜0.05).After 7,28,56 days of the intervention,muscle fiber cross-sectional area and diameter of the Liqi Buxue Decoction group were higher than those of the model group in the same period,the differences were statistically significant(P＜0.05).After 7,28,56 days of the intervention,collagen volume fraction of the gastrocnemius muscle of the model group and the Liqi Buxue Decoction group was lower than that of the sham operation group in the same period,the difference was statistically significant(P＜0.05).After 7,28,56 days of the intervention,collagen volume fraction of the gastrocnemius muscle of the Liqi Buxue Decoction group was lower than that of the model group in the same period,the difference was statistically significant(P＜0.05).After 7 days of the intervention,there was no statistically significant difference of Smad2 expression between the Liqi Buxue Decoction group and the sham operation group (P＞0.05).After 7,28,56 days of the intervention,the expression of TGF-β1,Smad2,Smad3,MyoD1 mRNA of the model group and the Liqi Buxue Decoction group were higher than those of the sham operation group in the same period,the differences were statistically significant(P＜0.05).After 7,28,56 days of the intervention,the expression of TGF-β1,Smad2,Smad3 mRNA were lower than those of the model group in the same period,the differences were statistically significant(P＜0.05).After 7,28,56 days of the intervention,the expression of MyoD1 mRNA of the Liqi Buxue Decoction group was higher than that of the model group in the same period,the difference was statistically significant(P＜0.05).After 7,28,56 days of the intervention,the expression of MURF1 mRNA of the model group and the Liqi Buxue Decoction group was higher than that of the sham operation group in the same period,the difference was statistically significant(P＜0.05).After 7,28,56 days of the intervention,the expression of MURF1 mRNA of the Liqi Buxue Decoction group was lower than that of the model group in the same period,the difference was statistically significant(P＜0.05).After 7,28,56 days of the intervention,the expression of phocnemius myosin of the model group and the Liqi Buxue Decoction group was lower than that of the sham operation group in the same period,the difference was statistically significant(P＜0.05).After 7,28,56 days of the intervention,the expression of phocnemius myosin of the Liqi Buxue Decoction group was lower than that of the model group in the same period,the difference was statistically significant(P＜0.05).Conclusion Liqi Buxue Decoction can delay denervated skeletal muscle atrophy in rats,which is related to regulating TGF-β1/Smad signal pathway,reducing skeletal muscle protein degradation and inhibiting skeletal muscle fibrosis.
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