Research of RhaFGF external application promotes healing of refractory skin ulcer in diabetic rats
SONG Jiuyu1 WANG Minhua2 LI Haikun2 LIAO Baochun2 CHEN Xiaobo3 Zhu Xiansen4 ZENG Xiangtai2▲
1. Department of General Surgery, the Second Hospital of Gannan Medical University, Jiangxi Province, Ganzhou 341000, China;
2. Department of Gastro-intestinal Surgery, the First Hospital of Gannan Medical University, Jiangxi Province, Ganzhou 341000, China;
3. Department of Repabilitation, the Second Hospital of Gannan Medical University, Jiangxi Province, Ganzhou 341000, China;
4. Department of Pathology, Gannan Medical University, Jiangxi Province, Ganzhou 341000, China
Objective To investigate the effect of recombinant human acidic fibroblast growth factor (RhaFGF) on healing of diabetic rats with refractory skin ulcers, and to further study the effect and mechanism of RhaFGF on the healing of diabetic rats with refractory skin ulcers. Methods The diabetic rat model of skin ulcer was established. A total of 28 rats were randomly divided into control group and RhaFGF group. The control group was treated with topical saline, and RhaFGF group was treated with topical application of RhaFGF. The wound area was recorded on the seventh day and the fourteenth day, and the new granulation tissue in the skin ulcer area of rats was collected. Superoxide dismutase (SOD), malondialdehyde (MDA), platelet endothelial cell adhesion molecule (CD31), functional antigen Ki-67 (Ki-67) and pathological sections were examined to evaluate the therapeutic effect of RhaFGF in treating diabetic refractory ulcers. Results On day 7 and 14 of treatment, the wound area of the RhaFGF group was smaller than that of the control group, and the difference was statistically significant (P<0.05). MDA content in the RhaFGF group was lower than that in the control group, and the difference was statistically significant (P<0.05). SOD activity in the RhaFGF group was higher than that in the control group, and the difference was statistically significant (P<0.05). The positive expression rate of CD31 in the RhaFGF group was higher than that in the control group, and the difference was statistically significant (P<0.01). The positive expression rate of Ki-67 in the RhaFGF group was higher than that in the control group, and the difference was statistically significant (P<0.01). Histopathological examination showed that the number of new capillaries, fibroblasts and collagen expression increased in RhaFGF group compared with before treatment. Conclusion RhaFGF promotes oxidative stress of diabetic refractory ulcers, increases the production of capillaries and fibroblasts in ulcerated tissues, and significantly increases the level of epithelial regeneration, thereby demonstrating the repair effect on diabetic ulcers.
Kimura K，Endo Y.Age-related changes in the tactile pressure sensitivity threshold in the sole of the foot in diabetic patients[J].J Phys Ther Sci，2018，30（7）：917-920.
[3]
Thangarajah H，Yao D，Chang EI，et al.The molecular basic for impaired hypoxia-induced VEGF expression in diabetic tissues[J].Proc Natl Acad Sci U S A，2019，106（32）：13505-13510.
[4]
Eming SA，Hammerschmidt M，Krieg T，et al.Interrelation of immunity and tissue repair or Regeneration[J].Semin Cell Dev Biol，2019，20（5）：517-527.
[5]
Su S，Cao M，Wu G，et al.Hordenine protects against hyperglycemia-associated renalcomplications in streptozocin-induced diabetic mice[J].Biomed Pharmacother，2018，104：315-324.
[6]
Herold C，Engeli S，Beckmann B，et al.Adipokine concentrations in lipoaspirates may have a role in wound healing[J].Indian J Plast Surg，2017，50（1）：56-63.
[7]
Kiec-Wilk B，Stolarz-Skrzypek K，Sliwa A，et al.Peripheral blood concentrations of TGF-β，IGF-1 and bFGF and remodelling of the left ventricle and blood vessels in hypertensive Patients[J].Kardiol Pol，2016，68（9）：996-1002.
[8]
Wu S，Wu Z，Zhu W，et al.Immunohistochemical study of the growth factors，aFGF，bFGF，PDGF-AB，VEGF-A and its receptor （Flk-l） during arteriogenesis[J].Mol Cell Biochem，2017，343（1-2）：223-229.
[10]
Wang W，Lin S，Xiao Y，et al.Acceleration of diabetic wound healing with chitosancrosslinked collagen sponge containing recombinant human acidic fibroblast growth factor in healing-impaired STZ diabetic rats[J].Life Sci，2008，82（3-4）：190-204.
[14]
Wong AK，Schonmeyr B，Singh P，et al.Histologic analysis of angiogenesis and lymphangiogenesis in acellular human dermis[J].Plast Reconstr Surg，2018，121（4）：1144-1152.
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