大黄素对海人酸致痫小鼠海马iNOS和COX-2表达的影响

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摘要目的探讨大黄素对海人酸诱导的小鼠癫痫持续状态后海马组织诱导型一氧化合酶（iNOS）、环氧化酶-2（COX-2）表达的影响。方法选取45只ICR雄性小鼠，随机分为对照组、癫痫持续状态组、大黄素治疗组，每组15只。采用海人酸建立小鼠癫痫模型，大黄素治疗组腹腔注射200 mg/kg的大黄素。癫痫持续状态组和对照组注入等量的生理盐水。致痫24 h后通过苏木精-伊红（HE）染色观察海马神经细胞的形态学变化，采用RT-PCR、Western blot分别检测iNOS、COX-2 mRNA与蛋白的表达。结果大黄素可明显改善癫痫鼠海马组织的病理形态学变化；大黄素治疗组iNOS、COX-2 mRNA与蛋白的含量明显低于癫痫持续状态组，差异有统计学意义（P＜0.05）。结论大黄素能降低小鼠癫痫持续状态后海马组织iNOS、COX-2 mRNA与蛋白的表达。

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	欧阳龙强
	夏文燕
	杨少春
	邹连生
	娄建云
	高志强

关键词 ：大黄素, 癫痫, iNOS, COX-2

Abstract：ObjectiveTo investigate Emodin′s influence on the expression of iNOS,COX-2 in hippocampus of mice after status epilepticus by kainic acid.Methods45 ICR male mice were selected and randomly divided into the control group,the SE group and the Emodin group,15 cases in each group.The epileptic model of mice was established by kainic acid.The treatment group was injected with 200 mg/kg by Emodin.The SE group and the control group were injected an equal amount of saline.HE stain was observed the morphological changes in hippocampal nerve after 24 h,RT-PCR and Western blot was used to detect the expression of the mRNA and protein level of iNOS,COX-2.ResultsEmodin could significantly relieve the hippocampal injury after status epilepticus in mice.The content of iNOS,COX-2 mRNA and protein in the Emodin group was significantly lower than that of the mRNA and protein in the SE group,with significant difference(P＜0.05).ConclusionEmodin may derease the expressions of the mRNA and protein 1evel of iNOS,COX-2 in hippocampus after status epilepticus in mice.

Key words： Emodin Epilepsy iNOS COX-2

基金资助:江西省赣州市指导性科技计划项目（GZ2016ZSF 020）

引用本文:

欧阳龙强;夏文燕;杨少春;邹连生;娄建云;高志强. 大黄素对海人酸致痫小鼠海马iNOS和COX-2表达的影响[J]. 中国当代医药, 2018, 25(2): 11-14.
OUYANG Long-qiang; XIA Wen-yan;YANG Shao-chun; ZOU Lian-sheng ;LOU Jian-yun; GAO Zhi-qiang. Influence of Emodin on the expression of iNOS,COX-2 in hippocampus after status epilepticus by kainic acid in mice. 中国当代医药, 2018, 25(2): 11-14.

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