探讨PCR-RFLP检测亚甲基四氢叶酸还原酶基因多态性与子痫前期发病机制的关系

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摘要目的探讨限制性片段长度多态性聚合酶链反应（cleaved amplification polymorphism sequence-tagged sites，PCR-RFLP）检测亚甲基四氢叶酸还原酶基因多态性与子痫前期发病机制的关系，为子痫前期的防治提供依据。方法选取2016年5月～2017年6月我院收治的子痫前期患者100例（病例组）、健康孕妇500例（对照组）作为研究对象，进行亚甲基四氢叶酸还原酶基因多态性分析、基因多态性检测，并进行血液生化指标分析，调查基本资料，进行因素分析。结果病例组患者所有测序都获得成功，对照组测序成功498例。两组患者的基因型频率、等位基因频率比较，差异有统计学意义（P＜0.05）。两组患者的初中及以下文化水平、有计划妊娠、有计划控制饮食与补充营养、增补叶酸、补充过维生素D、营养不良等因素的例数比较，差异有统计学意义（P＜0.05）。病例组患者的APPT低于对照组，Hcy高于对照组，差异有统计学意义（P＜0.05）。多因素分析采用Logistic回归分析，结果提示，等位基因TT、增补叶酸、孕期有计划的运动成为独立危险因素（P＜0.05），TT型与Hcy、APPT存在显著相关性（P＜0.05）。结论亚甲基四氢叶酸还原酶基因多态性与子癫前期有关且存在独立作用，可能与其可提高Hcy有关。

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	姜欣
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	李莉

关键词 ：子痫前期, GC基因, 基因多态性, 发病机制

Abstract：ObjectiveTo investigate the restriction fragment length polymorphism polymerase chain reaction(cleaved amplification polymorphism sequence-tagged sites,PCR-RFLP)in testing the relationship between methylenetetrahydrofolate reductase gene polymorphism and the pathogenesis of pre eclampsia,provide the basis for the prevention and treatment of preeclampsia.Methods100 preeclampsia (the case group)and 500 healthy pregnant women (the control group)from May 2016 to June 2017 treated in our hospital were selected as subjects to conduct polymorphism analysis of methylenetetrahydrofolate reductase gene and detect of gene polymorphism.Blood biochemical indexes were analyzed,basic data was investigated and factors were analyzed.ResultsAll cases were successfully sequenced in the case group,and 498 cases were successfully sequenced in the control group.The genotype frequency and allele frequency of the case group were compared,and the differences were statistically significant(P＜0.05).The the number of junior high school degree and below level,planned pregnancy,planned to control diet and nutritional supplements,folic acid,vitamin D supplements,the proportion of malnutrition were compared,and the differences were statistically significant(P＜0.05).APPT in the case group was lower than that in control group,and Hcy was higher than that in control group,the differences were statistically significant(P＜0.05).Logistic regression analysis was used for multivariate analysis.The results showed that allele TT,folic acid supplementation and planned exercise during pregnancy were independent risk factors(P＜0.05).There was significant correlation between TT type and Hcy and APPT(P＜0.05).ConclusionMethylenetetrahydrofolate reductase gene polymorphisms are associated with preeclampsia and have independent effects,which may be related to the elevation of Hcy.

Key words： Preeclampsia GC gene Gene polymorphism Pathogenesis

作者简介: 姜欣（1963-），女，山东济南人，本科，主任医师，研究方向：妇科内分泌和肿瘤

引用本文:

姜欣;舒俊俊;舒如明;龚丹;宴三华;李莉. 探讨PCR-RFLP检测亚甲基四氢叶酸还原酶基因多态性与子痫前期发病机制的关系[J]. 中国当代医药, 2017, 24(32): 86-89.
JIANG Xin;SHU Jun-jun;SHU Ru-ming;GONG Dan;YAN San-hua ;LI Li. To explore the PCR-RFLP in testing the relationship between methylenetetrahydrofolate reductase gene polymorphism and the pathogenesis of pre eclampsia. 中国当代医药, 2017, 24(32): 86-89.

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http://dangdaiyiyao.com/CN/ 或 http://dangdaiyiyao.com/CN/Y2017/V24/I32/86

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