白藜芦醇对血管紧张素Ⅱ诱导的平滑肌细胞增殖的抑制作用研究

摘要
图/表
参考文献(15)
相关文章 (4)

全文: PDF (0 KB) HTML (38 KB)
输出: BibTeX | EndNote (RIS)

摘要目的探讨白藜芦醇对血管紧张素Ⅱ诱导的平滑肌细胞增殖的抑制作用。方法体外培养大鼠胸主动脉血管平滑肌细胞（VSMCs），将细胞分为血管紧张素组、白藜芦醇梯度组、联合组及对照组，检测评价白藜芦醇和血管紧张素对VSMCs增殖能力的影响，各组均反应0、6、12、24 h，根据细胞计数来评价VSMCs的增殖情况，采用MTT比色法检验VSMCs活力，对结果进行对比分析。结果从各组的细胞计数结果来看，总体结果为血管紧张素组＞联合组＞白藜芦醇梯度组＞对照组，其中血管紧张素组VSMCs的细胞计数结果显著高于对照组，培养24 h时,白藜芦醇梯度组中30 μmol/L浓度下细胞计数最低（P＜0.05），而联合组中30、100 μmol/L浓度下细胞计数均显著低于10 μmol/L（P＜0.05）；从各组细胞活力情况来看，总体结果为血管紧张素组＞联合组＞白藜芦醇梯度组＞对照组,培养24 h时,同样白藜芦醇梯度组中30 μmol/L浓度下细胞计数最高（P＜0.05），而联合组中30、100 μmol/L浓度下细胞计数均显著低于10 μmol/L（P＜0.05）。结论白藜芦醇对于血管平滑肌细胞增殖有着一定的作用，且对于血管紧张素Ⅱ所诱导的增殖有着较强的抑制效果，不同浓度下作用效果有所差异。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	李自立
	章若涵

关键词 ：平滑肌细胞, 白藜芦醇, 血管紧张素Ⅱ, 细胞增殖

Abstract：ObjectiveTo explore inhibitory effect of Resveratrol on proliferation of smooth muscle cells induced by angiotensinⅡ.MethodsVascular smooth muscle cells(VSMCs)from rats were cultured in vitro.The cells were divided into angiotensin group,resveratrol gradient group,combined group and control group.The influence of resveratrol and angiotensin on proliferation of VSMCs was evaluated.The reaction on each group was lasted for 0,6,12,24 h,and proliferation of VSMCs was assessed by cell counting.The vitality of VSMCs was tested by MTT colorimetry and the results were compared and analyzed.ResultsWith regard to the results of cell counting,the general outcomes were angiotensin group, combination group,resveratrol gradient group,and control group from more to less,in which the cell counting of VSMCs in the angiotensin group was significantly higher than that in the control group (P＜0.05).In the resveratrol gradient group,the cell counting was lowest at 30 μmol/L when cultured for 24 h(P＜0.05).In the combination group,cell counting at 30 μmol/L and 100 μmol/L were greatly lower than that at 10 μmol/L(P＜0.05).Talking about cell viability,the general outcomes were angiotensin group,combination group,resveratrol gradient group,and control group from stronger to weaker.In the resveratrol gradient group,the cell counting was the highest at 30 μmol/L when cultured for 24 h (P＜0.05).In the combination group,the cell counting at 30 μmol/L and 100 μmol/L was both less than that at 10 μmol/L (P＜0.05).ConclusionResveratrol has a certain effect on proliferation of VSMCs,and has a strong inhibitory effect on proliferation induced by angiotensinⅡ.The effect differs in varied concentrations.

Key words： Smooth muscle cell Resveratrol AngiotensinⅡ Cell proliferation

作者简介: 李自立（1983-），男，湖南衡阳人，硕士，主治医师，主要研究方向：心血管内科

引用本文:

李自立;章若涵. 白藜芦醇对血管紧张素Ⅱ诱导的平滑肌细胞增殖的抑制作用研究[J]. 中国当代医药, 2017, 24(2): 10-12.
LI Zi-li;ZHANG Ruo-han. Inhibitory effect study of Resveratrol on proliferation of smooth muscle cell induced by angiotensinⅡ. 中国当代医药, 2017, 24(2): 10-12.

链接本文:

http://dangdaiyiyao.com/CN/ 或 http://dangdaiyiyao.com/CN/Y2017/V24/I2/10

[1]	Little PJ，Getachew R，Rezaei HB，et al.Genistein inhibits PDGF stimulated proteoglycan synthesis in vascular smooth muscle without blocking PDGF beta receptor phosphorylation[J].Arch Biochem Biophys，2012，525（1）：25-31.
[2]	Badimon L，Vilahur G.LDL-cholesterol versus HDL-cholesterol in the atherosclerotic plaque：inflammatory resolution versus thrombotic chaos[J].Ann N Y Acad Sci，2012，1254（2）：18-32.
[3]	Chu LM，Lassaletta AD，Robich MP，et al.Resveratrol in the prevention and treatment of coronary artery disease[J].Curr Atheroscler Rep，2011，13（6）：439-446.
[5]	郜攀，司良毅，徐强，等.白藜芦醇对血管紧张素Ⅱ诱导的血管平滑肌细胞增殖的抑制作用及其机制观察[J].解放军医学杂志，2013，38（4）：269-273.
[6]	李亮，许彦芳.AngⅡ对心肌细胞Kv4.2钾通道蛋白表达的影响及机制[J].武警医学院学报，2010，19（1）：26-28.
[9]	李春燕，江山，岳渝娟，等.分化抑制因子1对鼻咽癌细胞增殖的影响及其途径[J].解放军医学杂志，2012，37（6）：569-572.
[4]	PaffettML，Lucas SN，Campen MJ.Resveratrolreverses monocrotaline-induced pulmonary vascular and cardiac dysfunction：a potential role for atrogin-1 in smooth muscle[J]. Vascul Pharmacol，2012，56（1-2）：64-73.
[7]	Putnam K，Shoemaker R，Yiannikouris F，et al.The reninangiotensin system：a target of and contributor to dyslipidemias，altered glucose homeostasis，and hypertension of the metabolic syndrome[J].Am J Physiol Heart Circ Physiol，2012，302（6）：H1219-H1230.
[8]	Guo RW，Wang H，Gao P，et al.An essential role for stromal interaction molecule 1 in neointima formation following arterial injury[J].Cardiovasc Res，2009，81（4）：660-668.
[10]	冯帆，高旭东，陆荫英，等.LINE-1 ORF-1p对肝脏肿瘤细胞系和肝源永生化细胞系增殖的调控作用[J].解放军医学杂志，2012，37（3）：213-216.
[11]	贾庆华，杨霄鹏，惠玲，等.醋酸铅对人肾小球系膜细胞凋亡及Caspase-3表达的影响[J].解放军医学杂志，2012，37（6）：628-631.
[12]	Campbell KA，Lipinski MJ，Doran AC，et al.Lymphocytes and the adventitial immune response in atherosclerosis[J]. Circ Res，2012，110（6）：889-900.
[13]	Kroon PA，Iyer A，Chunduri P，et al.The cardiovascular nutrapharmacology of resveratrol：pharmacokinetics，molecular mechanisms and therapeutic potential[J].Curr Med Chem，2010，17（23）：2442-2455.
[14]	王智敏，吴瑕.阿托伐他汀对自发性高血压大鼠左心室肥厚的影响[J].郑州大学学报（医学版），2012，47（4）：554-556.
[15]	Schreiner CE，Kumerz M，Gesslbauer J，et al.Resveratrol blocks Akt activation in angiotensinⅡ-or EGF-stimulated vascular smooth muscle cells in a redox-independent manner[J].Cardiovasc Res，2011，90（1）：140-147.