微柱凝胶法交叉配血次侧弱凝集的原因及配血对策

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Abstract Objective To investigate the causes of weak agglutination on the secondary side of cross matching with micro-column gel test(MGT)and the countermeasures of matching.Methods A total of 3606 blood samples collected from September 2019 to October 2020 in Yunfu People′s Hospital were included in the study.The direct anti-human globulin test(DAT)was performed on the samples with weak agglutination on the secondary side of MGT cross-matching blood,and the antibody screening test was performed on donors.The causes of weak agglutination on the secondary side of MGT cross-matching blood were analyzed,and the corresponding matching countermeasures were discussed.Results There were 120 cases of secondary weak agglutination in the 3606 blood samples,including 117 cases of DAT positive,2 cases of positive donor antibody screening,and 1 case of false agglutination.Further immunophenotyping and diffusion tests were performed on the five DAT-positive specimens with obvious jaundice.The results showed that all five specimens were IgG-type,four were all negative for antibody screening after diffusion,and one was all positive for antibody screening after diffusion.Conclusion 97.50% of the cases of weak agglutination on the secondary side of MGT cross-matching blood are caused by DAT positivity in patients,and the agglutination intensity on the secondary side of cross-matching blood is not stronger than that of DAT in patients.For the specimen with DAT positive and obvious jaundice,in order to improve the safety and effectiveness of blood transfusion,the causes should be found and the corresponding blood matching scheme should be taken.

Key words： Micro-column gel test Cross-matching Weak agglutination on the secondary side Direct anti-human globulin test Blood transfusion

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	Articles by authors
	ZHU Jie-hao
	YE Huo-mei
	SUN Qin-jie

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ZHU Jie-hao,YE Huo-mei,SUN Qin-jie. Causes of weak agglutination on the secondary side of cross-matching with micro-column gel test and countermeasures of matching[J]. 中国当代医药, 2021, 28(17): 196-198.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2021/V28/I17/196

[1]	张印则，徐华，周华友.红细胞血型原理与检测策略[M].2 版.北京：人民卫生出版社，2019，182-183.
[2]	陈文静，郑宇浩.微柱凝集法在不规则抗体检测中的应用价值[J].国际检验医学杂志，2013，34（22）：3050.
[3]	Mark K.Fung，Brenda J.Grossman，Christopher D.Hillyer，等.AABB 技术手册[M].桂嵘，陈秉宇，黄远帅，等.18 版.长沙：中南大学出版社，2019：286-289.
[4]	阳福桂，刘芳，戚志萍，等.直接抗人球蛋白试验在临床贫血及输血患者中的应用[J].临床输血与检验，2015，17（6）：498-500.
[5]	戴小波，任燕歌，曾朱君，等.直接抗球蛋白试验阳性非自身免疫性溶血性贫血受血者的临床特征和输血对策[J].中国输血杂志，2013，26（6）：555-557.
[6]	林嘉，何屹，铙绍琴.微柱凝集技术交叉配血主侧相合、次侧不合的处理流程研究[J].国际检验医学杂志，2015，36（5）：581-585.
[7]	孙国常，张芸.直抗阳性导致配血不合的快速处置对策[J].中国输血杂志，2012，25（7）：695-696.
[8]	李翠莹，范秀.药物性抗体引起免疫性溶血反应的探讨[J].临床输血与检验，2018，20（1）：4-6.
[9]	Lin M，Wen YF，Wu JR，et al.Hemoglobinopathy: molecular epidemiological characteristics and health effects on Hakka people in the Meizhou region,southern China[J].PLoS One，2013，8（2）：e55024.
[10]	王琳，崔莹莹.RH 因子和HBS1L-MYB 基因多态性与重型β 地中海贫血患儿输血效果的关系探讨[J].实用预防医学，2019，26（12）：521-1524.
[11]	孙俊波，陆应玉.重型和中间型β-地中海贫血患儿输注洗涤红细胞的效果比较[J].临床输血与检验，2016，18（5）：446-448.
[12]	杨雪，谭春泽，李聚林.葡萄糖-6-磷酸脱氢酶缺乏症急性溶血与安全输血最新研究进展[J].中国实验血液学杂志，2018，26（5）：1569-1573.
[13]	危燕芬.广州某地区献血者G6PD 筛查情况分析[J].实验与检验医学，2017，35（3）：434-435.
[14]	冯志文，麦海，梁志东，等.取消微柱凝胶法次侧配血的可行性[J].国际检验医学杂志，2016，37（11）：1570-1571.
[15]	杨义中.次侧交叉配血必要性的探讨[J].实验与检验医学，2019，37（4）：760-761.
[16]	别立莉，梁会涛，安慧娟.抗体放散试验在直接抗人球蛋白试验阳性患者中的应用[J].实验与检验医学，2017，35（2）：288-290.