三联袋转移法在脐带血制备中的应用效果

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Abstract Objective To investigate the effect of tripe bag transfer method on the processing of cord blood total nucleated cells under aseptic surroundings.Methods A retrospective analysis of umbilical cord blood processing data from Guangzhou Cord Blood Bank from April 2019 to July 2020 and divided into traditional method group(267 cases)and transfer method group(278 cases)according to the separation method.The quality and efficiency of umbilical cord blood processing by the traditional secondary centrifugation method and the triple bag transfer method after transferring cord blood to empty bags and centrifuging were compared,and the quality indexes of cryopreservation volume,red blood cell removal rate,total nucleated cell number and recovery rate,CD34 positive cell number and progenitor cell colony number were compared between the two methods.Results The average cryopreservation volume of cord blood in the transfer method group was lower than that in the traditional method group,and the progenitor cell colonies in the transfer method group were higher than that in the traditional method group,and the differences were statistically significant(P＜0.05).There were no significant differences in the total number of nucleated cells,the recovery rate of nucleated cells,the depletion rate of red blood cells and the number of CD34 positive cells between the two groups(P>0.05).Conclusion The tripe bag transfer method is a simple,feasible alternative for umbilical cord blood processing without more cost,which is shows a practical value for product quality improvement in routine cord blood banking.

Key words： Umbilical cord blood Processing Triple bag transfer Volume reduction Cord blood banking

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	Articles by authors
	WU Jie-ying
	CHEN Jin-song
	WU Shao-qing
	LU Yan
	TANG Xue-wei
	LI Yan

Cite this article:

WU Jie-ying,CHEN Jin-song,WU Shao-qing, et al. Effect of triple bag transfer method on cord blood processing[J]. 中国当代医药, 2021, 28(15): 166-169.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2021/V28/I15/166

[1]	造血干细胞移植技术管理规范（2017年版）[S].国家卫生健康委员会，2017-02-04.
[2]	WMDA Global Trends Report 2019[DB/OL].[2020-09-13].https：//wmda.info/about-us/media-centre/
[3]	Statistics Search & Match Service-Total Number of Donors and Cord blood units[DB/OL].[2020-09-13].https：//wmda.info/about-us/media-centre/
[4]	Solves P，Mirabet V，Blanquer A，et al.A new automatic device for routine cord blood banking：critical analysis of different volume reduction methodologies[J].Cytotherapy，2009，11（8）：1101-1107.
[5]	Solves P，Mirabet V，Roig R.Volume reduction in routine cord blood banking[J].Curr Stem Cell Res Ther，2010，5（4）：362-366.
[6]	陈书勇，孙洪涛，李盼，等.脐带血质量管理发展与未来展望[J].母婴世界，2019，（17）：293.
[7]	Solves P，Planelles D，Mirabet V，et al.Qualitative and quantitative cell recovery in umbilical cord blood processed by two automated devices in routine cord blood banking：a comparative study[J].Blood Transfus，2013，11（3）：405-411.
[8]	吴洁莹，廖灿，许遵鹏，等.广州脐血库造血干细胞保存及临床应用[J].中华生物医学工程杂志，2010，16（5）：438-443.
[9]	吴春燕.基于脐带血造血干细胞分离冻存方法探究[J].健康必读，2019，（28）：25.
[10]	路春霞，魏本杰，舒畅，等.脐血单个核细胞分离方法的比较研究[J].临床血液学杂志，2016，29（7）：593-596.
[11]	吴秀娥，温素芬，王桂英.人脐带血采集数量和质量的影响因素分析[J].现代医院，2011，11（3）：21-23.
[12]	张晋，袁哲.影响脐带血采集质量的相关性因素分析[J].中华细胞与干细胞杂志（电子版），2018，8（4）：224-228.
[13]	Souri M，Nikougoftar Zarif M，Rasouli M，et al.Comparison of human umbilical cord blood processing with or without hydroxyethyl starch[J].Transfusion，2017，57（11）：2758-2766.
[14]	Schwandt S，Korschgen L，Peters S，et al.Cord blood collection and processing with hydroxyethyl starch or non-hydroxyethyl starch[J].Cytotherapy，2016，18（5）：642-652.
[15]	Patterson J，Moore CH，Palser E，et al.Detecting primitive hematopoietic stem cells in total nucleated and mononuclear cell fractions from umbilical cord blood segments and units[J].J Transl Med，2015，13：94.
[16]	Rich IN.Improving Quality and Potency Testing for Umbilical Cord Blood：A New Perspective[J].Stem Cells Transl Med，2015，4（9）：967-973.
[17]	Young，W.Plasma-depleted versus red cell-reduced umbilical cord blood[J].Cell Transplant，2014，23（4-5）：407-415.
[18]	王秋实，马良艳，张晋，等.1727份公共库脐带血的质量分析——附23份库存脐带血干细胞解冻质控分析[J].中国输血杂志，2014，27（12）：1317-1321.
[19]	杨俊晔，张亚斌，王福喜.干细胞分离仪与手工分离法分离脐带血的效果对比分析[J].现代诊断与治疗，2014，（7）：1641-1642.
[20]	]陈林，张坤，邵文陶，等.脐带血造血干细胞分离冻存方法优化[J].重庆理工大学学报（自然科学），2014，28（12）：82-86.
[21]	徐峰波，侯怀水，孙新伟，等.卡式分浆新技术在脐血制备中的应用[J].中国输血杂志，2013，26（11）：1116-1118.