封闭式薄片法在睾丸单精子冷冻保存中的应用探索

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Abstract Objective To investigate the effect of Closed-Sheet Method on testicular spermatozoa in patients with obstructive azoospermia(OA),in order to provide a simple and effective cryopreservation for individual numbers of testicular spermatozoa in patients with non-obstructive azoospermia(NOA).Methods 15 patients were diagnosed with OA by Reproductive Medicine & Genetic Center of Dalian from June to December 2016.Single testicular spermatozoa was frozen with Closed-Sheet Method,and we compared cleavage rate and fertility rate after ICSI between frozen-thawed individual numbers of testicular spermatozoa and fresh testicular sperm with abandoned in vitro maturation of immature oocytes,and the fertilization rate of frozen-thawed testicular spermatozoa was evaluated.Both frozen and fresh groups were from the above 15 patients.Results The frozen-thawed individual numbers of testicular spermatozoa had higher recovery rate and viability rate(92.00%,65.94%) with the Closed-Sheet Method.The fertility rate (56.00% vs 70.66%)and cleavage rate (97.44% vs 95.83%) of frozen testicular spermatozoa was similar to fresh testicular sperm after ICSI,and no significant difference(P>0.05).Conclusion Since the frozen-thawed testicular spermatozoa had a higher ability of fertilization.The Closed-Sheet Method was effective to cryopreserve individual numbers of testicular spermatozoa in patients with OA.This study laid the experimental basis for the clinical application of single sperm cryopreservation in patients with NOA.

Key words： Individual numbers testicular spermatozoa Cryopreservation Azoospermia Recovery rate

Received: 27 June 2017

PACS:

R321.33

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	MA Chao
	HU Ming-guang
	JIN Bo

Cite this article:

MA Chao,HU Ming-guang,JIN Bo. Application of the Closed-Sheet Method for individual numbers of testicular spermatozoa cryopreservation[J]. 中国当代医药, 2017, 24(24): 19-22.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2017/V24/I24/19

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