基于网络药理学探讨柚皮苷的药理作用机制

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Abstract Objective To explore the functional mechanism of naringin based on network pharmacology.Methods The Traditional Chinese Medicine Systems Pharmacology Database(TCMSP)was used to obtain naringin absorption,distribution,metabolism,and excretion properties evaluation,and target protein and genes of naringin were identified by GeneCards,and then were filtered out by Uniprot to select overlapping targets and genes.The analysis was performed through GeneMANIA database and Molecule Annotation System 3.0(MAS 3.0),including gene ontology(GO)enrichment and KEGG pathway analysis,and finally a visual network diagram was constructed through Cytoscape.Results Naringin had good druggability,and 14 putative protein targets were identified.Through enrichment analysis result,naringin mainly directly regulated bone metabolism through Wnt signaling pathways,TGF-β signaling pathway,MAPK signaling pathways and osteoclast differentiation signaling pathway,while through the P13K-Akt and VEGF signaling pathways affected bone metabolism indirectly to play the role of anti-osteoporosis.Its anti-cancer mechanism was mainly through influencing the endocrine inhibitory signaling pathway and the proteoglycan in cancer pathway.The anti-inflammatory and anti-oxidant mechanism was mainly through the intervention of MAPK signal pathway and TOLLlike receptor signaling pathway,etc.Conclusion The functional mechanism of naringin shows the characteristics of multiple targets.It plays a role in anti-cancer,regulating bone metabolism,anti-inflammatory and antioxidant process.The comprehensive application of network pharmacology can help to predict the pharmacological mechanism of compound quickly and comprehensively,and provide guidance for further experimental verification.

Key words： Network pharmacology Naringin Functional mechanism Gene ontology Pathway enrichment

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	CHEN De-yan

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CHEN De-yan. Exploration on the functional mechanism of naringin based on network pharmacology[J]. 中国当代医药, 2021, 28(2): 12-17.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2021/V28/I2/12

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