MG-132对TRAIL诱导骨肉瘤细胞U-2 OS凋亡的影响

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Abstract Objective To discusses the influence of MG-132 on TRAIL inducing apoptosis of osteosarcoma cell U-2OS.Methods Osteosarcoma cells U-2OS were cultured in 1640 culture medium containing 10%calf serum in a incubator at 37℃and 5%CO2saturation humidity.They were divided into single-use TRAIL group,single-use MG-132 group and combined group.After 24 hours of application of the above-mentioned three dosage regimens,and the apoptotic status of MG-132 cells was detected,the cell inhibition rate was calculated,and the cell changes were observed under light microscope.Results The effect of apoptosis induction in the single-use TRAIL group was not ideal.With the increase of TRAIL concentration,the apoptosis rate of U-2 OS of bone tumor was continuously increased.The apoptosis rate increased with the increase of drug concentration in single-use MG-132 group,and the difference was statistically significant(P＜0.05).The apoptotic rate of osteosarcoma cells in combined group,single-use TRAIL group and single-use MG-132 group was significantly higher than that in blank control group,and the difference were statistically significant(P＜0.05),and single-use MG-132 group was higher than single-use TRAIL group,and the difference was statistically significant(P＜0.05),and the apoptotic rate of osteosarcoma cells in combined group was significantly higher than that in single-use TRAIL group and single-use MG-132 group,and the difference was statistically significant(P＜0.05).With the passage of time,the cell inhibition rate increased,and the inhibition rate of combined group was significantly higher than that of single-use TRAIL group and single-use MG-132 group,the maximum value reached at 72 hours([82.32±2.54]%),and the difference was statistically significant(P＜0.05).Conclusion Single-use TRAIL can induce apoptosis of osteosarcoma cell U-2 OS,but the effect was not ideal and after combining use of MG-132,it can significantly improve the effect of TRAIL induced apoptosis of U-2 OS.

Key words： Ubiquitin-proteasome pathway TRAIL Osteosarcoma Apoptosis

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	FENG Dian-peng
	LIU Guang-hui
	HAN Qing

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FENG Dian-peng,LIU Guang-hui,HAN Qing. Influence of MG-132 on TRAIL inducing apoptosis of osteosarcoma cell U-2 OS[J]. 中国当代医药, 2019, 26(5): 12-15-19.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2019/V26/I5/12

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