二氢杨梅素对小鼠局灶性脑缺血/再灌注损伤的抗凋亡作用及机制研究

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Abstract Objective To study the anti-apoptosis effect of dihydromyricetin (DMY)on acute focal cerebral ischemia/reperfusion(I/R)injury in mice.Methods Male Kunming mice were randomly divided into sham group,I/R group and DMY group (500 mg/kg).Mice model of I/R injury was established by middle cerebral artery occlusion (MCAO)using modified thread method.Daily intragastric administration of DMY was carried out 10 days before the surgery,and 1 hour before ischemia and 12 hours after reperfusion.The brain tissues of the mice were harvested after ischemia for 3 hours and reperfusion for 24 hours.Myocyte apoptosis in the cerebral I/R brain was detected with in situ terminal deoxynucleotidyl transferase(TdT)-mediated dUTP nick-end labeling(TUNEL staining).The protein and mRNA expression of Bax and Bcl-2 were detected by immunohistochemistry assay and real-time quantitative PCR (RT-qPCR)respectively.Results Compared with sham group,apaotosis index was significantly higher,protein and mRNA expressions of Bcl-2 were lower than those of Bax were higher in I/R model group(all P＜0.01).Compared with I/R model group,in DMY group,apoptosis index decreased significantly,the expressions of Bcl-2 were higher,those of Bax were lower(all P＜0.01).Conclusion DMY can reduce the myocyte apoptosis induced by focal I/R injury,its anti-apoptotic mechanism might be related to regulating the expressionof Bax and Bcl-2.

Key words： Dihydromyricetin Cerebral ischemia/reperfusion injury Middle cerebral artery occlusion Apoptosis

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	Articles by authors
	GAN Lu
	DENG Zhi-jing
	WANG Xian-zhe
	SHU Qi
	LIANG Cong
	GUO Zhe
	HE Ping

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GAN Lu,DENG Zhi-jing,WANG Xian-zhe, et al. Study on anti-apoptosis effect and mechanism of dihydromyrcetin on focal cerebral ischemia-reperfusion injury in mice[J]. 中国当代医药, 2018, 25(14): 4-8.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2018/V25/I14/4

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