线粒体DNA缺失突变对顺铂耳毒易感作用的影响

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Abstract Objective To establish an animal model of mitochondrial DNA (mtDNA) deletion mutantion and to investigate the influence of mtDNA deletion mutantion on the ototoxicity susceptibility role of cisplatin.Methods Wistar rats were randomly divided into experimental group and control group，and there were 20 rats in each group.Doxorubicin (DOX) was subcutaneously injected at dose of 2 mg/kg twice per week for 3 months in experimental group.Control group was identical to experimental group，except normal saline was substituted for DOX.Cisplatin was intraperitoneally injected 2 mg/kg per day for 6 consecutive days in both groups.Auditory brainstem response (ABR) threshold was tested and 4834 bp deletion mutantion of mtDNA in inner ear membranous labyrinth for rats was tested by PCR technique.Results In experimental group，13 of the 20 rats were demonstrated 4834 bp mtDNA deletion，while control group was negative.ABR threshold was improved before and after cisplatin used in rats，and there was a statistical difference of mean between two groups (P＜0.05).Conclusion In the inner ear tissue of the rat，DOX can induce mtDNA deletion mutantion.mtDNA deletion of the inner ear tissue of the rat can increase its sensitivity of ototoxicity role of cisplatin.

Key words： mtDNA deletion Doxorubicin Ototoxicity susceptibility of cisplatin

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	Articles by authors
	LI Yu-fang
	YANG Zhi-hang
	YANG Yu
	NI Yue-qiu
	XING Ai-ping

Cite this article:

LI Yu-fang,YANG Zhi-hang,YANG Yu, et al. The influence of mtDNA deletion mutantion on the ototoxicity susceptibility of cisplatin[J]. 中国当代医药, 2016, 23(3): 102-104.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2016/V23/I3/102

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