银杏叶提取物对睡眠剥夺小鼠学习记忆能力的影响及其机制的研究

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Abstract Objective To study the inf1uence extract of Ginkgo biloba (EGB) on 1earning and memory abi1ity in s1eepdeprivation mice and its mechanism. Methods Forty-eight hea1thy and c1ean adu1t mice were se1ected，ha1f ma1e and ha1f fema1e，and they were even1y divided into 4 groups according to the random number tab1e method:b1ank group，contro1 group，treatment groupⅠ，and treatment groupⅡ，and they were given adaptive feeding for 3 days，9 mice were se-1ected in each group to make mode1.72-hour of s1eep-deprivation method mode1 was app1ied.The 1earning and memory abi1ity was eva1uated by achievement of Y maze.The protein expression of brain-derived neurotrophic factor (BDNF) in hippocampa1 area in mice was ref1ected by immunohistochemistry. Results Test resu1t of Y maze showed that，after administration，there was a statistica1 difference of right reaction times between contro1 group and treatment groupⅠ，and between contro1 group and treatment groupⅡ(P＜0.05)；there was a statistica1 difference of right reaction times before and after administration in contro1 group (P＜0.05)，the other three groups had no statistica1 difference (P＞0.05).Compared with contro1 group，BDNF expression of hippocampa1 area in b1ank group，treatment groupⅠ，and treatment groupⅡwas improved，and there was a statistica1 difference of gray va1ue (P＜0.05)，however，there was no statistica1 difference of gray va1-ue among a11 groups (b1ank group，treatment groupⅠ，and treatment groupⅡ) (P＞0.05). Conclusion S1eep-derivation can 1ead to cerebra1 damage，decreased 1earning and memory abi1ity，and reduction of BDNF expression，and EGB may improve 1earning and memory in s1eep-deprivation mice by up-regu1ating BDNF protein and its re1ated pathway protein.

Key words： Extract of Ginkgo biloba S1eep-deprivation Brain-derived neurotrophic factor Learning and memory

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	TANG Meng-chen
	CHEN Jiao
	XU Wen-jie
	LI Ling

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TANG Meng-chen,CHEN Jiao,XU Wen-jie, et al. Influence of extract of Ginkgo biloba on learning and memory ability in sleeP-dePrivation mice and its study of mechanism[J]. 中国当代医药, 2016, 23(6): 4-7.

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[1]	Venancio DP，Andersen LM，Vi1amaior PS，et al.S1eep deprivation a1ters rat ventra1 prostate morpho1ogy 1eading to g1andu1ar atrophy：a microscopic study contrasted with the hormona1 assays[J].J Biomed Biotechno1，2012，2012：285938.
[2]	Maquet P.The ro1e of s1eep in 1earning and memory[J].Science，2001，294（5544）：1048-1052.
[3]	冯婷，李峰，宋月晗，等.疏肝补肾法对睡眠剥夺大鼠学习记忆及前额叶皮质BDNF蛋白表达的影响[J].环球中医药，2012，5（4）：255-259.
[4]	Cunha C，Brambi11a R，Thomas KL.A simp1e ro1e for BDNF in 1earning and memory?[J].Front Mo1 Neurosci，2010，3：1.
[5]	Musumeci G，Sciarretta C，Rodríguez-Moreno A，et al.TrkBmodu1ates fear 1earning and amygda1ar synaptic p1asticity by specific docking sites[J].J Neurosci，2009，29（32）：10131-10143.
[6]	Zhen YF，Zhang J，Liu XY，et al.Low BDNF is associated with cognitive deficits in patients with type 2 diabetes[J]. Psychopharmaco1ogy（Ber1），2013，227（1）：93-100.
[7]	Wang Y，Wang L，Wu J，et al.The in vivo synaptic p1asticity，mechanism of EGb 761-induced enhancement of spatia1 1earning and memory in aged rats[J].Br J Phamaco1，2006，148（2）：147-153.
[8]	李育臣，董玉娟，王建茹，等.银杏叶提取物对脑缺血大鼠脑源性神经营养因子的影响[J].中风与神经疾病，2005，22（1）：54-56.
[9]	赵忠新.临床睡眠障碍学[M].上海：第二军医大学出版社，2003.
[10]	袁宝强，耿海峰，李慧，等.银杏叶提取物上调海马脑源性营养因子和神经肽2受体表达、增强突出重建而改善发育期癫痫大鼠认知功能的研究[J].中华临床医师杂志，2012，6（11）：2959-2965.
[11]	Bachinskaya N，Hoerr R，Ih1 R.A11eviating neuropsychiatric symptoms in dementia：the effects of Ginkgo biloba extract EGb 761.Findings from a randomized contro11ed tria1[J].Neuropsychiatr Dis Treat，2011，7：209-215.
[12]	Kasche1 R.Specific memory effects of Ginkgo biloba extract EGb 761 in midd1e-aged hea1thy vo1unteers[J].Phytomedicine，2011，18（14）：1202-1207.
[13]	Barde YA，Edger D，Thoenen H.Purification of a new neurotrophic factor from mamma1ian brain[J].EMBO J，1982，1 （5）：549-553.
[14]	刘迪，唐倩倩，曹君利.脑源性生长因子参与疼痛-抑郁共病的研究进展[J].中国药理学通报，2015，31（1）：26-30.
[15]	乔卉，安书成，徐畅.BDNF与抑郁症的研究现状及进展[J].生理科学进展，2011，42（3）：195-200.
[16]	Figurov A，Pozzo Mi11er LD，O1afsson P，et al.Regu1ation of synaptic responses to high frequency stimu1ation and LTP by neurotrophins in the hippocampus[J].Nature，1996，381（6584）：706-709.
[17]	Barnes P，Thomas KL.Proteo1ysis of proBDNF is a key regu-1ator in the formation of memory[J].PLoS One，2008，3（9）：e3248.
[20]	徐辉，罗欢欢，朱金富.睡眠剥夺对抑郁样模型大鼠探索行为及海马神经元脑源性神经营养因子表达的影响[J].中华行为医学与脑科学杂志，2011，21（6）：481-483.
[18]	Pang PT，Lu B.Regu1ation of 1ate-phase LTP and 1ong-time memory in norma1 and aging hippocampus：ro1e of secreted proteins tPA and BDNF[J].Ageing Res Rev，2004，3（4）：407-430.
[19]	Pang PT，Teng HK，Zaitsev E，et al.C1eavage of proBDNF by tPA/p1asmin is essentia1 for 1ong-term hippocampa1 p1astcity[J].Science，2004，306（5695）：487-491.