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| Research on ultrasonic extraction process of total Flavonoids from the roots of Ephedra sinica Stapf |
| YU Qi |
| Department of Pharmacy,Central Hospital of Loudi City in Hunan Province,Loudi 417000,China |
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Abstract Objective To study the extraction process of the total Flavonoids from roots of Ephedra sinica Stapf.Methods The content of total Flavonoids in root of Ephedra sinica Stapf was determined by UV visible spectrophotometry.The influence of ethanol fraction,solid-liquid ratio,extraction duration and extraction times on the extraction rate of total flavonoids were investigated,and the orthogonal design was used to optimize the extraction process.Results The detection wavelength was 500 nm.The RSD of precision and reproducibility tests were 1.26% and 1.07%,the sample was stable within 10 h,and the average recovery rate was 98.80%.The optimum conditions for extracting the total flavonoids were as follow:material-liquid ratio was 1∶12,alcohol concentration was 75%,extraction times was twice and extraction duration was 30 min.Conclusion The extraction process is simple,stable and feasible,and suitable for the industrial production.
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Received: 19 June 2017
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