香烟烟雾提取物对小鼠C2C12细胞myostatin及炎症介质的影响

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Abstract ObjectiveTo investigate the effect of cigarette smoke extract on myostatin and inflammatory mediators in mouse C2C12 cells.MethodsDifferent concentrations of CSE and/or myostatin-siRNA stimulated or interference mouse C2C12 cells,and then detected influence of CSE on cell growth by MTT,according to the different treatment methods,the experimental research objects were divided into the control group,5%CSE group,the myostatin-siRNA group,5%CSE＋myostatin-siRNA group.Real-time PCR was used to detect the expression of myostatin mRNA,Western blot was used to detect expression of myostatin protein,ELISA was used to detected tumor necrosis factor alpha (TNF-alpha)and interleukin 8(IL-8)level in cell culture medium.ResultsThe CSE at the concentration above 5%could significantly inhibit the proliferation of C2C12 cells in mice(P＜0.05).The expression of myostatin mRNA and protein in C2C12 cells treated with 5%CSE was significantly higher than that of the control group after 48 h(P＜0.05).The content of IL-8 and TNF-alpha in the cell culture fluid was significantly higher than that in the control group (P＜0.05).After siRNA interfered with myostatin,the mouse C2C12 cells were treated with CSE,the content of IL-8 and TNF-alpha in cell culture solution was significantly lower than that in CSE treatment group(P＜0.05).ConclusionCSE can up-regulate the expression of myostatin and increase inflammatory mediators releasing from C2C12 cells.

Key words： Chronic obstructive pulmonary disease Cigarette smoke extract Myostatin Inflammatory mediator

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	Articles by authors
	LI Yu-ji
	ZHOU Xu
	PEI Xing-hua
	HUANG Li
	LEI Yan-jun
	ZHANG Lu-lu

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LI Yu-ji,ZHOU Xu,PEI Xing-hua, et al. Influence of cigarette smoke extract on myostatin and inflammatory mediators in mouse C2C12 cells[J]. 中国当代医药, 2018, 25(5): 4-7.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2018/V25/I5/4

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