N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸对大鼠肝纤维化模型跨膜信号转导的影响

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Abstract ObjectiveTo observe the effect of N-acetyl-seryl-aspartyl-lysyl-proline(AcSDKP)on rat liver fibrosis due to bile duct ligation (BDL),and to explore the relationship between this effect and TGF-β1-Smad2/3 and BMP-7-Smad1/5/8 signal channels.Methods30 SD rats were selected and randomly divided into the sham operation group,the model group and the treatment group(AcSDKP),BDL liver fibrosis model was built.when making model of the treatment group,subcutaneous micropump was implanted to provide AcSDKP treatment.On the 14thday,the rats were killed,then ELISA Enzyme linked immunosorbent assays were adopted to detect the hyaluronic acid(HA)and CollagenⅢ(PCⅢ) level,hydroxyproline(Hyp)measurement method was adopted to analyze the content of total collagen in hepatic tissue in a quantitative manner.Western blotting was adopted to detect the expression of transforming growth factor-β1(TGF-β1), bone morphogenetic protein-7(BMP-7),phosphorylation Smad2/3 and phosphorylation Smad1/5/8 protein in hepatic tissue.ResultsCompared with the sham operation group,the serum levels of HA,PCⅢ,and content of Hyp in hepatic tissue in the model group were increased(P＜0.05).The serum levels of HA,PCⅢ,and content of Hyp in hepatic tissue in the treatment group was significantly lower than that in the model group,with significant difference(P＜0.01).The expression of TGF-β1,phosphorylation Smad2/3 in the model group was higher than that in the sham operation group,with significant difference(P＜0.05).The expression of TGF-β1,phosphorylation Smad2/3 in the treatment group was lower than that in the model group,the expression of BMP-7 phosphorylation Smad1/5/8 in the treatment group was higher than that in the model group,with significant difference(P＜0.05).ConclusionAcSDKP has the effect of inhibiting BDL liver fibrosis in rats,which may be related to the inhibition of TGF-beta 1 pathway and activation of BMP-7 pathway.

Key words： N-acetyl-seryl-aspartyl-lysyl-proline Transforming growth factor-beta 1;Bone morphogenetic protein-7;Liver fibrosis

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	ZHANG Lei
	LIU Hong-fu
	WU Xiong-jian

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ZHANG Lei,LIU Hong-fu,WU Xiong-jian. Influence of N-acetyl-seryl-aspartyl-lysyl-proline on transmembrane signal transduction of rat liver fibrosis model[J]. 中国当代医药, 2016, 23(35): 4-6转14.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2016/V23/I35/4

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