GICA检测登革热病毒NS1抗原和(或)IgG/IgM抗体的临床意义

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Abstract ObjectiveTo analyze and explore the effect of application of colloidal gold immunochromatography assay (GICA)in the detection of Dengue virus NS1 antigen and/or IgG/IgM antibody.MethodsAltogether 1200 cases of suspected Dengue virus infection patients who were treated in our hospital from January 2014 to June 2015 were involved as the object of this research and divided into two groups(PCR group and ELISA group)by using the random grouping method,with 600 casesin each group.Two groups were treated with polymerase chain reaction(PCR)fluorescence probe and enzyme-linked immunosorbent assay(ELISA),respectively.The two types of samples aforementioned were randomly divided into 3 sub-groups,with 200 samples in each sub-groups:GICA was used in group A to detect NSI antigen;GICA was used in group B to detect IgG/IgM antibody;it was also used in group C for detection of NS1 antigen and IgG/IgM antibody.Additionally,60 healthy volunteers were selected as Group D,whose NS1 antigen and IgG/IgM antibody were detected by using GICA.ResultsELISA group results:compared with the results of ELISA group,the positive,negative and overall coincidence rate of group A were 82.88%,90.00%and 83.00%,with statistically significant difference(P<0.05);compared with the results of ELISA group,the positive,negative and overall coincidence rate of group B were 88.20%,71.79%and 85.00%,with statistically significant difference(P<0.05);compared with the results of ELISA Group,the positive,negative and overall coincidence rate of group A were 94.87%,47.73%and 84.50%,without statistically significant difference(P>0.05);PCR group results:compared with the results of PCR group,the positive,negative and overall coincidence rate of group A were 87.68%,83.87% and 86.50%,with statistically significant differenc(P< 0.05);compared with the results of PCR group,the positive,negative and overall coincidence rate of groupB were 91.45%,85.42%and 90%,with statistically significant difference(P<0.05);compared with the results of PCR Group,the positive,negative and overall coincidence rate of Group B were 95.43%,60.00%and 91.00%,without statistically significant difference(P>0.05).The result of Group D was negative and its specificity was 100%.ConclusionThe result achieved by application of GICA is close to that of ELISA and PCR in detection of NIS and IgG/IgM with good specificity.tcan be used for early screening and diagnosis of dengue virus infections.

Key words： Colloidal gold immunochromatographic assay Enzyme-linked immunosorbent assay Polymerase chain reaction Dengue virus

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	Articles by authors
	ZHONG Xiu-yin
	YANG Xiao-bin
	HE Xiang-juan
	HUANG Shao-xia
	CAI Yi

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ZHONG Xiu-yin,YANG Xiao-bin,HE Xiang-juan, et al. Clinical significance of GICA in detection of Dengue virus NS1 antigen and/or IgG/IgM antibody[J]. 中国当代医药, 2017, 24(12): 156-158转163.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2017/V24/I12/156

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