双黄连注射液和双黄连冻干粉针剂对家兔离体红细胞溶血作用的影响

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Abstract ObjectiveTo observe the influence of Shuanghuanglian Injection and Shuanghuanglian Lyophilized Power Injection on hemolytic effect in rabbits′red blood cell(RBC)in vitro.MethodsThe blood samples were collected from healthy rabbits by 20 ml,and the red blood cell suspension of 2%was prepared,and it with Shuanghuanglian Injection, Shuanghuanglian Lyophilized Power Injection and Sodium Chloride Injection of 0.9%were co-incubated,and hemolysis test was carried out in vitro,and the absorbance was detected by spectrophotometer.The hemolysis rate of RBC in 3 h was observed.ResultsThe hemolysis rate of Shuanghuanglian Injection with concentration of 0.06,0.12,0.18,0.24,0.3 g/ml was 8.39%,21.34%,40.98%,50.36%,67.68%respectively,and there was a statistical difference between two groups compared with Sodium Chloride Injection of 0.9%(P＜0.05).The hemolysis rate of Shuanghuanglian Lyophilized Power Injection with concentration of 0.06,0.12,0.18,0.24,0.3 g/ml was 2.15%,5.23%,7.31%,9.59%,13.35%respectively,and there was a statistical difference when the concentration was 0.3 g/ml compared with Sodium Chloride Injection of 0.9%(P＜0.05).When the concentration was 0.12,0.18,0.24,0.3 g/ml,there was a statistical difference of hemolysis rate between Shuanghuan glian Injection and Shuanghuanglian Lyophilized Power Injection(P＜0.05).ConclusionThe influence of Shuanghuanglian Lyophilized Power Injection on hemolytic effect is small compared with Shuanghuanglian Injection．

Key words： Shuanghuanglian Injection Shuanghuanglian Lyophilized Powder Injection Hemolysis test

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	Articles by authors
	SHANG Shu-yu
	HU Xiao-feng
	LI Jing
	QIN Jun-lian

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SHANG Shu-yu,HU Xiao-feng,LI Jing, et al. The influence ofShuanghuanglian Injection and Shuanghuanglian Lyophilized Power Injection on hemolytic effect in rabbits′red blood cells in vitro[J]. 中国当代医药, 2017, 24(12): 147-149.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2017/V24/I12/147

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