口服pCDNA3.1+/Ag85A DNA疫苗在小鼠肠道的表达

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Abstract ObjectiveTo analyze the expression of oral pCDNA3.1+/Ag85A DNA vaccine in the intestinal tract of mice.MethodsOral vaccine was made using liposomes to parcel pCDNA3.1+/Ag85A DNA plasmid.5 female C57BL/6 mice in control group were given saline water for gavage while 5 female C57BL/6 mice in experimental group were given liposome encapsulated Ag85A DNA vaccine for gavage.The mice were immunized three times with 14 days interval between each time.In the fourteenth day after immunization,cervical dislocation executed in mice and the small intestine was fixed with 4%paraformaldehyde to be prepared to detect the expression of Ag85A in the intestinal Peyer patches,the dendritic cells in Peyer patches and intestinal epithelial cells with immunofluorescence and immunohistochemistry methods.ResultsOral vaccine pCDNA3.1+/Ag85A DNA was expressed in intestinal Peyer patches,the dendritic cells in Peyer patches and the intestinal epithelial cells of mice in the experimental group,and in different parts of the intestinal epithelial cells in mice of the experimental group,the expression intensity of Ag85A was different.The expression intensity of Ag85A was higher near the lamina propria than near the lumen of intestinal epithelial cells(P＜0.05).It was not expressed in above parts of mice in the control group.ConclusionOral DNA vaccine parceled with liposomes can be absorbed by intestinal tract of mice and induce specific mucosal immune response.

Key words： Ag85A DNA vaccine Liposomes Epithelial cells Dendritic cells Mice

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	XU Jia
	LIU Ying
	YU Miao
	FENG Yong-hui
	LYU Chang-long

Cite this article:

XU Jia,LIU Ying,YU Miao, et al. Expression of Oral pCDNA3.1+/Ag85A DNA vaccine in the intestine of mice[J]. 中国当代医药, 2017, 24(18): 4-7.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2017/V24/I18/4

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