骨髓间质干细胞移植后肝星状细胞凋亡情况及其治疗肝纤维化的机制探讨

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Abstract ObjectiveTo investigate the apoptosis of hepatic stellate cells after bone marrow mesenchymal stem cell transplantation and its mechanism in the treatment of hepatic fibrosis.Methods30 clean SD rats were purchased from Animal Experimental Center,Zhongshan University,and 20 of them were prepared for experimental research.Only ten rats whose bone marrow mesenchym stem cells were extracted and selected as the experimental group for the establishment of rat model with hepatic fibrosis.The hepatic fibrosis and apoptosis of hepatic stellate cells were intervened by bone marrow stem cell mobilization to test these indices.Another group without extraction of bone marrow mesenchymal stem cells was classified into the control group(n=10),which was not performed with intervention.The purity identification of bone marrow mesenchymal stem cells was analyzed,and the hydroxyproline content as well as hepatic stellate cell activation and apoptosis were statistically analyzed.ResultsThe percentage of CD34,CD44,CD45 and CD95 in negative cells were 99.75%,99.51%,99.80%and 95.72%,respectively.After 8-week induction via subcutaneous injection of CCl4,the content of hydroxyproline in the rat′s liver in the experimental group was significantly higher than that before transplantation(P＜0.05).In the experimental group,the content of hydroxyproline in the liver after one-week bone marrow mesenchymal stem cell transplantation was much lower compared with that 3-day after transplantation(P＜0.05).In the control group,the content of hydroxyproline in the liver after one-weekbone marrow mesenchymal stem cell transplantation was greatly higher in comparison with that 3-day after transplantation(P＜0.05).The content of hydroxyproline in the liver after one-week bone marrow mesenchymal stem cell transplantation in the experimental group was remarkably lower than that in the control group(P＜0.05).The amount of hepatic stellate positive cells in the experimental group one-week after bone marrow mesenchymal stem cell transplantation was greatly less than that in the control group(P＜0.05).The amount of hepatic stellate apoptotic cells 3-day after bone marrow mesenchymal stem cell transplantation in the experimental group was remarkably more than that before transplantation and in the control group(P＜0.05).ConclusionThe hepatic stellate cell apoptosis induced by bone marrow mesenchymal stem cells may be a major mechanism for the treatment of hepatic fibrosis.

Key words： Bone marrow mesenchymal stem cell transplantation Hepatic stellate cells Apoptosis Hepatic fibrosis Treatment mechanism

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	Articles by authors
	WU Xiong-jian
	ZHENG Hong
	ZHU Hai-yan
	MAO Zhong-yi
	ZHANG Lei
	XIE Jun

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WU Xiong-jian,ZHENG Hong,ZHU Hai-yan, et al. Exploration of the apoptosis of hepatic stellate cells after bone marrow mesenchymal stem cell transplantation and its mechanism in the treatment of hepatic fibrosis[J]. 中国当代医药, 2017, 24(21): 107-110.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2017/V24/I21/107

[1]	何文艳，刘树贤，姜慧卿.大鼠骨髓间充质干细胞向类肝细胞体外诱导分化[J].世界华人消化杂志，2008，16（22）：2464-2469.
[2]	焦艳，朱月永，郑琦，等.骨髓间充质干细胞移植对大鼠肝纤维化的作用[J].肝脏，2008，13（1）：23-26.
[3]	史立军，王菁华，徐克达，等.骨髓基质干细胞对大鼠肝纤维化细胞凋亡的影响[J].中国生物制品学杂志，2008，21（11），929-932.
[4]	闫磊，李思源，慕晓玲.SD大鼠骨髓间充质干细胞分离培养及其生物学特性鉴定[J].实用医学杂志，2008，24（17）：2945-2947.
[5]	卢仁荣，陈良龙，江琼，等.大鼠骨髓间充质干细胞的分离培养纯化与鉴定[J].心肺血管病杂志，2008，27（4）：239-242.
[6]	胡昆鹏，林楠，林继宗，等.人骨髓间质干细胞对肝星状细胞的体外调控[J].中国组织工程研究与临床康复，2009，13（27）：5257-5260.
[7]	Qin S，Jiang H，Su S，et al.Inhibition of hepatic stellate cell proliferation by bone marrow mesenchymal stem cells via regulation of the cell cycle in rat[J].Exp Ther Med，2012，4（3）：375-380.
[8]	Troeger JS，Mederacke I，Gwak GY，et al.Deactivation of hepatic stellate cells during liver fibrosis resolution in mice[J].Gastroenterology，2012，143（4）：1073-1083.
[9]	毕研梅.慢性乙型肝炎患者天门冬氨酸氨基转移酶/血小板指数在肝纤维化分级中的临床意义[J].中国当代医药，2015，22（2）：43-44.
[10]	吴雄健，毛忠懿.TGF-1-Smad3-ILK信号转导通路与大鼠肝纤维化的相关性研究[J].中国当代医药，2013，20（36）：14-15.
[11]	Christ B，Dollinger MM.The generation of hepatocytes from mesenchymal stem cells and engraftment into the liver[J].Curr Opin Organ Transplant，2011，16（1）：69-75.
[12]	Hsu CS，Liu WL，Chao YC，et al.Adipocytokines and liver fibrosis stages in patients with chronic hepatitis B virus infection[J].Hepatol Int，2015，9（2）：231-242.
[13]	Yang G，Bo C，Chen L，et al.Current status and future prospects of mesenchymal stem cell therapy for liver fibrosis[J].J Zhejiang Univ Sci B，2016，17（11）：831-841.
[14]	AttallahAM，El-FarM，OmranMM，etal.Levelsofschistosoma mansoni circulating antigen in chronic hepatitis C patients with different stages of liver fibrosis[J].J Immunoassay and Immunochem，2016，37（3）：316-330.
[15]	Kazutaka T，Kojiro T，Yukinori K，et al.Migration of splenic lymphocytes promotes liver fibrosis through modification of T helper cytokine balance in mice[J].J Gastroenterol，2015，50（10）：1054-1068.