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Exploration of the apoptosis of hepatic stellate cells after bone marrow mesenchymal stem cell transplantation and its mechanism in the treatment of hepatic fibrosis |
WU Xiong-jian1 ZHENG Hong2 ZHU Hai-yan1 MAO Zhong-yi1 ZHANG Lei1 XIE Jun1 |
1.Department of Gastroenterology,the First Affiliated Hospital of Gannan Medical University,Jiangxi Province,Ganzhou 341000,China;
2.Gannan Medical University,Jiangxi Province,Ganzhou341000,China |
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Abstract ObjectiveTo investigate the apoptosis of hepatic stellate cells after bone marrow mesenchymal stem cell transplantation and its mechanism in the treatment of hepatic fibrosis.Methods30 clean SD rats were purchased from Animal Experimental Center,Zhongshan University,and 20 of them were prepared for experimental research.Only ten rats whose bone marrow mesenchym stem cells were extracted and selected as the experimental group for the establishment of rat model with hepatic fibrosis.The hepatic fibrosis and apoptosis of hepatic stellate cells were intervened by bone marrow stem cell mobilization to test these indices.Another group without extraction of bone marrow mesenchymal stem cells was classified into the control group(n=10),which was not performed with intervention.The purity identification of bone marrow mesenchymal stem cells was analyzed,and the hydroxyproline content as well as hepatic stellate cell activation and apoptosis were statistically analyzed.ResultsThe percentage of CD34,CD44,CD45 and CD95 in negative cells were 99.75%,99.51%,99.80%and 95.72%,respectively.After 8-week induction via subcutaneous injection of CCl4,the content of hydroxyproline in the rat′s liver in the experimental group was significantly higher than that before transplantation(P<0.05).In the experimental group,the content of hydroxyproline in the liver after one-week bone marrow mesenchymal stem cell transplantation was much lower compared with that 3-day after transplantation(P<0.05).In the control group,the content of hydroxyproline in the liver after one-weekbone marrow mesenchymal stem cell transplantation was greatly higher in comparison with that 3-day after transplantation(P<0.05).The content of hydroxyproline in the liver after one-week bone marrow mesenchymal stem cell transplantation in the experimental group was remarkably lower than that in the control group(P<0.05).The amount of hepatic stellate positive cells in the experimental group one-week after bone marrow mesenchymal stem cell transplantation was greatly less than that in the control group(P<0.05).The amount of hepatic stellate apoptotic cells 3-day after bone marrow mesenchymal stem cell transplantation in the experimental group was remarkably more than that before transplantation and in the control group(P<0.05).ConclusionThe hepatic stellate cell apoptosis induced by bone marrow mesenchymal stem cells may be a major mechanism for the treatment of hepatic fibrosis.
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