尼莫地平预处理对烧伤大鼠认知功能的影响

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Abstract Objective To investigate the effect of Nimodipine on cognitive function in burned rats and its mechanism.Methods A total of 32 healthy male SD rats aged 18 months were selected.The rats were divided into four groups (n=24) by random number table method: control group (group C),surgery group (group S),Nimodipine group (group D),Nimodipine + surgery group (group D+S).Groups D and D+S were intraperitoneally injected with 1mg/kg Nimodipine,while groups C and S were given the same amount of normal saline;30 min later,the group S and group D+S were anesthetized and burned models were created.Group C was the blank control group.Morris water maze method was used to record the escape latency and crossing times of rats 1 day before operation and the 1st,3rd,7th days after operation.After completing,the Morris water maze,the rats were sacrificed and their hippocampus were collected.The tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the hippocampus were detected by ELISA.MRNA expressions of hippocampal Bax and Bcl-2 were determined by RT-PCR.The apoptosis rate of hippocampal neurons was measured by flow cytometry. Results Compared with 1 day before the operation,there were no significant differences in the average escape latent period and crossing times between group C and group D on the 1st,3rd,and 7th days after operation(P＞0.05).Compared with group C，the average escape latent period of group S and D+S was prolonged and the crossing times were reduced on the 1st，3rd and 7th days after operation (P＞0.05).Compared with group S ,the average escape latent period of the group D+S at the same time was shortened and the crossing times at the same time were increased on the 1st，3rd and 7th days (P<0.05).Compared with group S,the levels of TNF-α and IL-6 in the hippocampus of the rats in the group D+S were decreased,the mRNA expression of Bax in hippocampal neurons was reduced,the mRNA expression of Bcl-2 was increased,the ratio of Bax/Bcl-2 was reduced,and the apoptosis rate was decreased (P<0.05).Conclusion Nimodipine can improve postoperative cognitive dysfunction in burned elderly rats by inhibiting inflammatory response and decreasing hippocampal neuron apoptosis.

Key words： Nimodipine Apoptosis rate Cognitive function Burned

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	LIU Tian-lin
	QU Min

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LIU Tian-lin,QU Min. Effect of Nimodipine pretreatment on cognitive function in burned rats[J]. 中国当代医药, 2020, 27(21): 12-15 转21.

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https://dangdaiyiyao.com/EN/ OR https://dangdaiyiyao.com/EN/Y2020/V27/I21/12

[1]	Skvarc DR，Berk M，Byrne LK，et al.Post-Operative Cognitive Dysfunction：An exploration of the inflammatory hypothesis and novel therapies[J].Neurosci Biobehav Rev，2018，84（11）：116-133.
[2]	Zenilman ME.Delirium：an important postoperative complication[J].JAMA，2017，317（1）：77-78.
[3]	李茂华，滕苗，果磊.PI3K/Akt 信号通路在严重烧伤大鼠血清诱导BMSCs 迁移中的作用[J].重庆医学，2015，44（27）：3752-3755.
[4]	Palmu R，Suominen K，Vuola J，et al.Mental disorders among acute burn patients[J].Burns，2010，36（7）：1072-1079.
[5]	王黎丽，郭松雪，吴攀，等.富氢盐水对重度烧伤大鼠急性肾损伤的影响和机制[J].中华烧伤杂志，2018，34（9）：629-636.
[6]	Biala G，Kruk-Slomka M，Jozkwiak K.Influence of acute or chroniccalcium channel antagonists on the acquisition and consolidation of memory and nicotine-indeced cognitive effects in mice[J].Naunyn-Schmied Arch Pharmacol，2013，386（7）：651-664.
[7]	王光毅，朱世辉，唐洪泰，等.尼莫地平对大鼠烫伤后库普弗细胞白介素-1β 和白介素-6 产生的抑制作用[J].中国危重病急救医学，2003，15（4）：210-212.
[8]	冯世杰，华兰女，金曙雯.大鼠烫伤模型的制作[J].上海第二医科大学学报，1995，16（3）：195-197.
[9]	Chen Y，Lei Y，Mo LQ，et al.Electroacupuncture pretreatment with different waveforms prevents brain injury in rats subjected to cecal ligation and puncture via inhibiting microglial activation，and attenuating inflammation，oxidative stress and apoptosis[J].Brain Res Bull，2016（10）127：248-259.
[10]	Rappold T，Laflam A，Hori D，et al.Evidence of an association between brain cellular injury and cognitive decline after non-cardiac surgery[J].Br J Anaesth，2016，116（1）：83-89.
[11]	Fan CH，Peng B，Zhang FC.The postoperative effect of sevoflurane inhalational anesthesia on cognitive function and inflammatory response of pediatric patients[J].Eur Rev Med Pharmacol Sci，2018，22（12）：3971-3975.
[12]	Qi Z，Tianbao Y，Yanan L，et al.Pre-treatment with nimodipine and 7.5% hypertonic saline protects aged rats against postoperative cognitive dysfunction via inhibiting hippocampal neuronal apoptosis[J].Behav Brain Res，2017，321（3）：1-7.
[13]	陈旭光，何金华，李亚南，等.电针改善老龄大鼠术后认知功能障碍的机制与海马神经元线粒体途径凋亡的关系[J].中华麻醉学杂志，2019，39（1）：40-43.
[14]	袁天宝，王秋筠.尼莫地平预先给药对老龄大鼠术后认知功能障碍的影响[J].中华麻醉学杂志，2014，34（9）：1054-1057.
[15]	Vorhees CV，Williams MT.Morris water maze: procedures for assessing spatial and related forms of learning and memory[J].Nat Protoc，2006，1（2）：848-858.
[16]	Zhang Q，Li Y，Bao Y，et al.Pretreatment with nimodipine reduces incidence of POCD by decreasing calcineurin mediated hippocampal neuroapoptosis in aged rats[J].BMC Anesthesiol，2018，18（1）：42-49.
[17]	周鑫，高欣欣，陈欣欣，等.大面积烧伤引起谵妄的研究进展[J].中华损伤与修复杂志（电子版），2014，10（2）：180-184.
[18]	吴林，唐农，麻小梅，等.温肺降浊方对血管性痴呆大鼠海马CA1 区神经元凋亡及Bcl-2、Bax 蛋白表达的影响[J].中国老年学杂志，2019，39（10）：5074-5077.