秦艽不同醇提物的抑菌作用比较

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Abstract Objective To analyze the in vitro antimicrobial activities of different ethanol extracts from Gentianae Macrophyllae Radix to Staphylococcus aureus,Staphylococcus epidermidis,Enterococcus faecalis and Shigella flexneri.Methods Oxford cup method and two fold dilution test tube combined with agar plate method were used to determine the antibacterial activities in vitro and minimal inhibitory concentration (MIC) of different extracts with ethanol,65% ethanol and water as solvent of Gentianae Macrophyllae Radix on four kinds of bacteria.Results Three kinds of ethanol extracts of Gentianae Macrophyllae Radi had certain antibacterial effects on bacteria,and the 65% ethanol extract was the best one,which antibacterial activities to Enterococcus faecium and Staphylococcus epidermidis were stronger and MIC was 7.83 g/L and 15.63g/L separately.MIC of Staphylococcus aureus was 62.50 g/L.Conclusion 65% extracts of Gentianae Macrophyllae Radi have a stronger antibacterial activity for Enterococcus faecium,Staphylococcus epidermidis and Staphylococcus aureus,which provides the experimental basis for the rational use of this kind of medicinal herbs.

Key words： Gentianae Macrophyllae Radix Antibacterial activity Minimal inhibitory concentration Comparison

Received: 05 July 2017

PACS:

R286.0

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	Articles by authors
	LI Jun
	WANG Hong-yan
	ZHANG Ran
	HAO Cai-qin

Cite this article:

LI Jun,WANG Hong-yan,ZHANG Ran, et al. Comparison of antibacterial activities of different ethanol extracts from Gentianae Macrophyllae Radix[J]. 中国当代医药, 2017, 24(25): 130-132.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2017/V24/I25/130

[1]	聂安政,林志健,王雨,等.秦艽化学成分及药理作用研究进展[J].中草药,2017,48（3）：597-608.
[2]	国家药典委员会.中国药典[M].北京：中国医药科技出版社,2015.
[3]	Jiang ZB,Liu HL,Liu XQ, et al .Chemical constituents of Gentiana macrophylla Pall[J].Nat Prod Res,2010,24（14）：1365-1369.
[4]	Wei SH,Zhang PC,Feng XZ, et al .Qualitative and quantitative determination of ten iridoids and secoiridoids in Gentiana straminea Maxim. by LC-UV-ESI-MS[J].J Nat Med,2012,66（1）： 102-108.
[5]	王长生,董红娇,包雅婷,等.UPLC-Q-Exactive四级杆-静电场轨道阱高分辨质谱联用快速分析粗茎秦艽化学成分[J].中草药,2016,47（18）：3175-3180.
[6]	中华本草编委会.中华本草（第17卷）[M].上海：上海科学技术出版社,1999.
[7]	张鹏,刘瑢,张霞,等.秦艽对小鼠酒精性肝损伤的保护作用[J].中国医院药学杂志,2014,34（21）：1822-1825
[8]	王佳,王钢,王丽琴,等.秦艽醇提物治疗类风湿关节炎的实验研究[J].西部中医药,2015,28（7）：11-14.
[9]	Zhao L,Ye J,Wu GT, et al .Gentiopicroside prevents interleukin-1 beta induced inflammation response in rat articular chondrocyte[J].J Ethnopharmacol,2015,172：100-107.
[10]	李娅,赵锡兰,杨凤琴,等.秦艽醇提取物对8种细菌的体外抑菌实验[J].中国医院药学杂志,2011,31（23）：1940-1942.
[11]	赵兵,赵婷,高昂,等.3种秦艽药材提取物的抑菌作用比较[J].西北大学学报（自然科学版）,2012,42（6）：971-974.
[12]	叶文初,袁富威,班贇.苦豆草提取物对奶牛乳房炎致病菌的体外抑菌作用[J].中国兽医学报,2016,36（9）：1568-1571.
[13]	张立明,张霞,杨凤琴.黄连与痢特灵联用体外抑菌试验研究[J].中国现代应用药学,2010,27（6）：482-494.
[14]	罗宇东,张强,蒋林,等.常春卫矛提取物体外及体内抑菌作用的试验研究[J].中国当代医药,2014,21（18）：9-11.
[15]	张振巍,张娜娜,崔璀,等.淫羊藿不同提取物的体外抑菌实验研究[J].中国药师,2016,19（12）：2247-2250.
[16]	左春连,韩成云,刘智凯,等.抗菌消炎妇科中药的筛选及临床前实验研究[J].中国当代医药,2016,23（36）：121-124.
[17]	汪学军,闵长莉,韩彭垒.博落回不同部位提取物对大肠菌群的抑菌作用研究[J].天然产物研究与开发,2016,28（2）：247-250.
[18]	穆祯强,于洋,高昊,等.龙胆属秦艽组植物的化学成分和药理作用研究进展[J].中国中药杂志,2009,34（16）：2012-2017.