|
|
|
| Study on quality standard of sugarless Waiganfengsha Granules |
| LUO Yu-dong1,2,3, LU Shi-heng1, TAN An-qiang1, WU Yu-qiang1,3, CHEN Hong-tao1▲ |
1.Pharmaceutical Factory,Guangxi Traditional Chinese Medical University,Nan-ning 530023,China;
2.Key Laboratory of Zhuang Medicine Prescriptions Basis and Application Research of Guangxi Colleges and Universities,Nanning 530001,China;
3.Key Laboratory of Generic Technology Development of Chinese Medicine Preparation of Guangxi Colleges and Universities,Nanning 530001,China |
|
|
|
|
Abstract Objective To set up the quality control standard of sugarless Waiganfengsha Granules.Methods Thin layer chromatography (TLC) was used,and petroleum ether (60-90℃)-ethyl acetate-glacial acetic acid (16:2:1) was the developing agent,and 10% sulfuric acid ethanol solution selected as the chromogenic agent to qualitatively identify Streptocaulon griffithii.Toluene-ethyl acetate-glacial acetic acid (10:3:1) was the developing agent,and it was observed under ultraviolet lamp (365 nm) to qualitatively identify Holly root.HPLC was used,chromatographic column:Shimadzu C18 chromatographic column (4.6 mm×150 mm,5 μm) was adopted,and mobile phase:acetonitrile-0.4% phosphoric acid solution (13:87),current speed:0.5 ml/min,and column temperature:25℃,detection wavelength was 327 nm,sample size:10 μl,and sampling time was 60 min.The content of chlorogenic acid in the product was determined.Results According to TLC method,in test sample chromatography,in the corresponding position with Streptocaulon griffithii chromatogram of contrast medicinal material,1 or more than 1 spots in same color were displayed while in the corresponding position with Holly root chromatogram of contrast medicinal material 1 or more than 1 bright spots in same color were displayed,and the spots were clear,and the specificity was strong with good reproducibility,and it had no interference of negative.Chlorogenic acid had a good linear relationship with peak area in the range of 6.2968-201.5 μg (r=0.9991) of sample size,and the average recovery rate was 100.11%,and RSD was 0.14% (n=6).The average content of Chlorogenic acid was 1.1069 mg/g (RSD=0.45 %) among 10 batches of sample tablets.Conclusion The quality control method used has a characteristics of sensitive method,strong specificity,good reproducible and high stability,and it can be used as the quality standard control method of sugarless Waiganfengsha Granules.
|
|
Received: 23 June 2017
|
|
|
|
|
|
| [1] |
蒋林,李茂,黎明,等.外感风痧片对风热感冒的药效学研究[J].中国药师,2010,13（1）：57-58.
|
| [2] |
蒋林,李茂,黎明,等.外感风痧片体内抑菌、抗病毒作用的试验研究[J].世界中医药,2010,5（1）：72-74.
|
| [3] |
张玉军.外感风痧片对大鼠的长期毒性研究[J].中成药,2009,31（2）：287-290.
|
| [4] |
林启云,李爱媛,周芳,等.外感风痧颗粒（片）剂药理研究[J].广西中医学院学报,2001,4（4）：88-90.
|
| [5] |
覃学谦,刘源焕,陈洪涛.正交试验优选无糖外感风痧颗粒一步制粒工艺[J].广西中医药,2015,38（5）：79-80.
|
| [6] |
刘源焕,吴玉强,蒋林,等.正交试验优选无糖外感风痧颗粒的成型工艺[J].中国实验方剂学杂志,2011,17（3）：11-13.
|
| [7] |
谭珍媛,雷震鸣.正交试验法优选外感风痧颗粒制剂的提取工艺[J].广西中医学院学报,2007,10（3）：76-78.
|
| [8] |
张琳,王叶飞,徐丽珍.藤苦参化学成分研究[J].中国药学杂志,2007,42（6）：24-26.
|
| [9] |
彭霞,王志杰,林艳芳.傣药藤苦参质量标准研究[J].中国民族民间医药,2009,18（1）：30-31.
|
| [10] |
栾连军,王叶飞,张琳.藤苦参素的体外抗肿瘤活性及其对癌细胞凋亡的作用[J].药学学报,2007,42（1）：104-107.
|
| [11] |
朱华,刘安韬,廖月葵.古羊藤的显微鉴别研究[J].广西中医学院学报,2007,10（1）：55-57.
|
| [12] |
杜冰曌,杨鑫瑶,冯晓,等.岗梅的化学成分和药理作用研究进展[J].中国中药杂志,2017,42（1）：20-27.
|
| [13] |
郑夏生,袁玉贤,徐晖,等.岗梅药材的TLC鉴别和HPLC指纹图谱[J].中国实验方剂学杂志,2013,19（21）：123-126.
|
| [14] |
彭敏桦,张敏敏,沈雅婕,等.岗梅总皂苷含量测定方法建立及不同部位含量比较研究[J].中医药导报,2015,21（3）：29-30.
|
| [15] |
王美琴,徐圣秋,雷雨,等.HPLC法测定不同采收期苍耳草中绿原酸的含量[J].现代中药研究与实践,2011,25（6）：83-85.
|
| [16] |
王仕平,刘惠民,谢勇,等.苍耳草的质量标准研究[J].中国医院用药评价与分析,2014,14（3）：220-222.
|
| [17] |
仝俊太,徐圣秋,李伟东,等.高效液相色谱法测定苍耳草中绿原酸的含量[J].中国医院药学杂志,2011,31（20）：1739-1740.
|
| [18] |
尹靖先,邓晓鸿,车晓彦,等.苍耳子的薄层层析鉴别研究[J].华西药学杂志,2005,20（1）：67-69.
|
| [19] |
杨柳,吴金雄,许舜军,等.苍耳子中酚酸类化合物的鉴别及绿原酸的含量测定[J].中国实验方剂学杂志,2011, 17（19）：85-88.
|
|
|
|
